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Crystal structure of Escherichia coli YidC, a membrane protein chaperone and insertase

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dc.contributor.author Kumazaki, Kaoru en
dc.contributor.author Kishimoto, Toshiki en
dc.contributor.author Furukawa, Arata en
dc.contributor.author Mori, Hiroyuki en
dc.contributor.author Tanaka, Yoshiki en
dc.contributor.author Dohmae, Naoshi en
dc.contributor.author Ishitani, Ryuichiro en
dc.contributor.author Tsukazaki, Tomoya en
dc.contributor.author Nureki, Osamu en
dc.date.accessioned 2015-05-26T08:06:17Z en
dc.date.available 2015-05-26T08:06:17Z en
dc.date.issued 2014-12-03 en
dc.identifier.issn 20452322 en
dc.identifier.uri http://hdl.handle.net/10061/9861 en
dc.description.abstract Bacterial YidC, an evolutionally conserved membrane protein, functions as a membrane protein chaperone in cooperation with the Sec translocon and as an independent insertase for membrane proteins. In Gram-negative bacteria, the transmembrane and periplasmic regions of YidC interact with the Sec proteins, forming a multi-protein complex for Sec-dependent membrane protein integration. Here, we report the crystal structure of full-length Escherichia coli YidC. The structure reveals that a hydrophilic groove, formed by five transmembrane helices, is a conserved structural feature of YidC, as compared to the previous YidC structure from Bacillus halodurans, which lacks a periplasmic domain. Structural mapping of the substrate- or Sec protein-contact sites suggested the importance of the groove for the YidC functions as a chaperone and an insertase, and provided structural insight into the multi-protein complex. en
dc.language.iso en en
dc.publisher Macmillan Publishers en
dc.rights 2014 Macmillan Publishers Limited. All Rights Reserved. en
dc.subject Structural biology en
dc.subject X-ray crystallography en
dc.title Crystal structure of Escherichia coli YidC, a membrane protein chaperone and insertase en
dc.type.nii Journal Article en
dc.textversion Publisher en
dc.identifier.ncid AA10703873 en
dc.identifier.jtitle SCIENTIFIC REPORTS en
dc.identifier.volume 4 en
dc.relation.doi 10.1038/srep07299 en
dc.identifier.artnum 7299 en
dc.identifier.NAIST-ID 73299737 en

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