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Longevity Regulation by Proline Oxidation in Yeast

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dc.contributor.author Nishimura, Akira en
dc.contributor.author Yoshikawa, Yuki en
dc.contributor.author Ichikawa, Kazuki en
dc.contributor.author Takemoto, Tetsuma en
dc.contributor.author Tanahashi, Ryoya en
dc.contributor.author Takagi, Hiroshi en
dc.date.accessioned 2021-12-16T00:55:39Z en
dc.date.available 2021-12-16T00:55:39Z en
dc.date.issued 2021-08-02 en
dc.identifier.uri http://hdl.handle.net/10061/14576 en
dc.description.abstract Proline is a pivotal and multifunctional amino acid that is used not only as a nitrogen source but also as a stress protectant and energy source. Therefore, proline metabolism is known to be important in maintaining cellular homeostasis. Here, we discovered that proline oxidation, catalyzed by the proline oxidase Put1, a mitochondrial flavin-dependent enzyme converting proline into ∆1-pyrroline-5-carboxylate, controls the chronological lifespan of the yeast Saccharomyces cerevisiae. Intriguingly, the yeast strain with PUT1 deletion showed a reduced chronological lifespan compared with the wild-type strain. The addition of proline to the culture medium significantly increased the longevity of wild-type cells but not that of PUT1-deleted cells. We next found that induction of the transcriptional factor Put3-dependent PUT1 and degradation of proline occur during the aging of yeast cells. Additionally, the lifespan of the PUT3-deleted strain, which is deficient in PUT1 induction, was shorter than that of the wild-type strain. More importantly, the oxidation of proline by Put1 helped maintain the mitochondrial membrane potential and ATP production through the aging period. These results indicate that mitochondrial energy metabolism is maintained through oxidative degradation of proline and that this process is important in regulating the longevity of yeast cells. ja
dc.language.iso en en
dc.publisher MDPI en
dc.rights © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). ja
dc.subject chronological lifespan en
dc.subject energy metabolism en
dc.subject homeostasis en
dc.subject longevity en
dc.subject proline en
dc.subject proline oxidase en
dc.subject Saccharomyces cerevisiae en
dc.title Longevity Regulation by Proline Oxidation in Yeast en
dc.type.nii Journal Article en
dc.contributor.transcription ニシムラ, アキラ ja
dc.contributor.transcription ヨシカワ, ユウキ ja
dc.contributor.transcription イチカワ, カズキ ja
dc.contributor.transcription タケモト, テツマ ja
dc.contributor.transcription タナハシ, リョウヤ ja
dc.contributor.transcription タカギ, ヒロシ ja
dc.contributor.alternative 西村, 明 ja
dc.contributor.alternative 吉川, 雄樹 ja
dc.contributor.alternative 市川, 和希 ja
dc.contributor.alternative 武本, 哲磨 ja
dc.contributor.alternative 棚橋, 亮弥 ja
dc.contributor.alternative 高木, 博史 ja
dc.textversion publisher en
dc.identifier.eissn 2076-2607 en
dc.identifier.jtitle Microorganisms en
dc.identifier.volume 9 en
dc.identifier.issue 8 en
dc.relation.doi 10.3390/microorganisms9081650 en
dc.identifier.NAIST-ID 82040965 en
dc.identifier.NAIST-ID 83515130 en
dc.identifier.NAIST-ID 87642054 en
dc.identifier.NAIST-ID 87643433 en
dc.identifier.NAIST-ID 84362144 en
dc.identifier.NAIST-ID 73290561 en
dc.relation.pmid 34442729 en
dc.relation.isIdenticalTo https://www.mdpi.com/2076-2607/9/8/1650 en

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