DSpace Repository

Cysteine residues in the fourth zinc finger are important for activation of the nitric oxide-inducible transcription factor Fzf1 in the yeast Saccharomyces cerevisiae

Show simple item record

dc.contributor.author Nasuno, Ryo en
dc.contributor.author Yoshioka, Natsuko en
dc.contributor.author Yoshikawa, Yuki en
dc.contributor.author Takagi, Hiroshi en
dc.date.accessioned 2021-09-13T02:35:16Z en
dc.date.available 2022-07-10 en
dc.date.issued 2021-07-10 en
dc.identifier.uri http://hdl.handle.net/10061/14449 en
dc.description.abstract Nitric oxide (NO) is a ubiquitous signaling molecule in various organisms. In the yeast Saccharomyces cerevisiae, NO functions in both cell protection and cell death, depending on its concentration. Thus, it is important for yeast cells to strictly regulate NO concentration. The transcription factor Fzf1, containing five zinc fingers, is reportedly important for NO homeostasis by regulating the expression of the YHB1 gene, which encodes NO dioxygenase. However, the mechanism by which NO activates Fzf1 is still unclear. In this study, we showed that NO activated Fzf1 specifically at the protein level by RT-qPCR and Western blotting. Our further transcriptional analyses indicated that cysteine residues in the fourth zinc finger (ZF4) are required for the NO-responsive activation of Fzf1. Additionally, the present results suggest that ZF4 is important for the protein stability of Fzf1. From these results, we proposed possible mechanisms underlying Fzf1 activation. en
dc.language.iso en en
dc.publisher Wiley en
dc.relation.isversionof https://onlinelibrary.wiley.com/doi/10.1111/gtc.12885?af=R en
dc.rights © 2021 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd This is the peer reviewed version of the following article: [https://onlinelibrary.wiley.com/doi/10.1111/gtc.12885?af=R], which has been published in final form at [https://doi.org/10.1111/gtc.12885]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. 出版社許諾条件により、本文は2022年07月11日以降に公開 ja
dc.subject nitric oxide en
dc.subject Saccharomyces cerevisiae en
dc.subject transcription factor Fzf1 en
dc.subject yeast en
dc.subject zinc finger en
dc.title Cysteine residues in the fourth zinc finger are important for activation of the nitric oxide-inducible transcription factor Fzf1 in the yeast Saccharomyces cerevisiae en
dc.type.nii Journal Article en
dc.contributor.transcription ナスノ, リョウ ja
dc.contributor.transcription ヨシカワ, ユウキ ja
dc.contributor.transcription タカギ, ヒロシ ja
dc.contributor.alternative 那須野, 亮 ja
dc.contributor.alternative 吉川, 雄樹 ja
dc.contributor.alternative 高木, 博史 ja
dc.textversion author en
dc.identifier.eissn 1365-2443 en
dc.identifier.jtitle Genes to Cells en
dc.relation.doi 10.1111/gtc.12885 en
dc.identifier.NAIST-ID 82045303 en
dc.identifier.NAIST-ID 83515130 en
dc.identifier.NAIST-ID 73290561 en
dc.relation.pmid 34245655 en

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search


My Account