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Effect of the Ala234Asp replacement in mitochondrial branched-chain amino acid aminotransferase on the production of BCAAs and fusel alcohols in yeast

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dc.contributor.author Koonthongkaew, Jirasin en
dc.contributor.author Toyokawa, Yoichi en
dc.contributor.author Ohashi, Masataka en
dc.contributor.author Large, Christopher R. L. en
dc.contributor.author Dunham, Maitreya J. en
dc.contributor.author Takagi, Hiroshi en
dc.date.accessioned 2021-09-10T01:20:08Z en
dc.date.available 2021-09-10T01:20:08Z en
dc.date.issued 2020-08-10 en
dc.identifier.uri http://hdl.handle.net/10061/14444 en
dc.description.abstract In the yeast Saccharomyces cerevisiae, the mitochondrial branched-chain amino acid (BCAA) aminotransferase Bat1 plays an important role in the synthesis of BCAAs (valine, leucine, and isoleucine). Our upcoming study (Large et al. bioRχiv. 10.1101/2020.06.26.166157, Large et al. 2020) will show that the heterozygous tetraploid beer yeast strain, Wyeast 1056, which natively has a variant causing one amino acid substitution of Ala234Asp in Bat1 on one of the four chromosomes, produced higher levels of BCAA-derived fusel alcohols in the brewer’s wort medium than a derived strain lacking this mutation. Here, we investigated the physiological role of the A234D variant Bat1 in S. cerevisiae. Both bat1∆ and bat1A234D cells exhibited the same phenotypes relative to the wild-type Bat1 strain—namely, a repressive growth rate in the logarithmic phase; decreases in intracellular valine and leucine content in the logarithmic and stationary growth phases, respectively; an increase in fusel alcohol content in culture medium; and a decrease in the carbon dioxide productivity. These results indicate that amino acid change from Ala to Asp at position 234 led to a functional impairment of Bat1, although homology modeling suggests that Asp234 in the variant Bat1 did not inhibit enzymatic activity directly. ja
dc.language.iso en en
dc.publisher Springer en
dc.relation.isversionof https://link.springer.com/article/10.1007%2Fs00253-020-10800-y en
dc.rights © 2020, Springer-Verlag GmbH Germany, part of Springer Nature The original publication is available at https://link.springer.com/ ja
dc.subject Yeast en
dc.subject Saccharomyces cerevisiae en
dc.subject Mitochondrial branched-chain amino acid aminotransferase Bat1 en
dc.subject Branched-chain amino acids en
dc.subject Fusel alcohols en
dc.subject Beer brewing en
dc.title Effect of the Ala234Asp replacement in mitochondrial branched-chain amino acid aminotransferase on the production of BCAAs and fusel alcohols in yeast en
dc.type.nii Journal Article en
dc.contributor.transcription トヨカワ, ヨウイチ ja
dc.contributor.transcription タカギ, ヒロシ ja
dc.contributor.alternative 豊川, 洋一 ja
dc.contributor.alternative 高木, 博史 ja
dc.textversion author en
dc.identifier.eissn 1432-0614 en
dc.identifier.jtitle Applied Microbiology and Biotechnology en
dc.identifier.volume 104 en
dc.identifier.issue 18 en
dc.identifier.spage 7915 en
dc.identifier.epage 7925 en
dc.relation.doi 10.1007/s00253-020-10800-y en
dc.identifier.NAIST-ID 86630464 en
dc.identifier.NAIST-ID 74654492 en
dc.identifier.NAIST-ID 73290561 en
dc.relation.pmid 32776205 en

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