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Characterization of collagenase found in the nonpathogenic bacterium Lysinibacillus sphaericus VN3

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dc.contributor.author Bach, Thi Mai Hoa
dc.contributor.author Pham, Thanh Huyen
dc.contributor.author Dinh, Truong Son
dc.contributor.author Takagi, Hiroshi
dc.date.accessioned 2021-09-10T00:18:39Z
dc.date.available 2021-09-10T00:18:39Z
dc.date.issued 2020-11-01
dc.identifier.uri http://hdl.handle.net/10061/14443
dc.description.abstract High collagenolytic activity has been detected in pathogenic bacteria. Collagenase plays an essential role in the invasion step in animals and humans. In this study, we characterized collagenase found in the nonpathogenic bacterium Lysinibacillus sphaericus VN3, which was isolated from soil in Vietnam. The collagenase activity of the purified enzyme was strongly inhibited by Cu2+, but it was significantly increased by Zn2+. The purified enzyme with a molecular mass of approximately 110 kDa exhibited collagenolytic, gelatinolytic, and caseinolytic activity. The kinetic studies showed that this enzyme had greater hydrolyzing activity toward collagen and gelatin compared with casein. Based on the ratio Vmax/Km, collagen is likely to be the best substrate among three proteins. We found that this collagenase could digest small pieces of bovine skin and tendon into a collagen solution. Interestingly, at pH 6.0–8.0, the soluble collagen could form a collagen membrane, which is useful as a wound-healing biomaterial. ja_JP
dc.language.iso en ja_JP
dc.publisher 日本農芸化学会 ja_JP
dc.relation.isversionof https://academic.oup.com/bbb/article-abstract/84/11/2293/6044524?redirectedFrom=fulltext
dc.rights © 2020 Japan Society for Bioscience, Biotechnology, and Agrochemistry This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 01 November 2020, available online: http://www.tandfonline.com/10.1080/09168451.2020.1799748 出版社許諾条件により、本文は2021年11月02日以降に公開 ja_JP
dc.subject Bacterial collagenase ja_JP
dc.subject metalloproteinase ja_JP
dc.subject Lysinibacilus sphaericus ja_JP
dc.title Characterization of collagenase found in the nonpathogenic bacterium Lysinibacillus sphaericus VN3 ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription タカギ, ヒロシ
dc.contributor.alternative 高木, 博史
dc.textversion author ja_JP
dc.identifier.eissn 1347-6947
dc.identifier.jtitle Bioscience, Biotechnology, and Biochemistry ja_JP
dc.identifier.volume 84 ja_JP
dc.identifier.issue 11 ja_JP
dc.identifier.spage 2293 ja_JP
dc.identifier.epage 2302 ja_JP
dc.relation.doi 10.1080/09168451.2020.1799748 ja_JP
dc.identifier.NAIST-ID 73290561 ja_JP
dc.relation.pmid 32741269 ja_JP

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