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Inhibitory effect of arginine on proline utilization in Saccharomyces cerevisiae

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dc.contributor.author Nishimura, Akira
dc.contributor.author Tanikawa, Tsubasa
dc.contributor.author Takagi, Hiroshi
dc.date.accessioned 2021-09-08T02:28:03Z
dc.date.available 2021-09-08T02:28:03Z
dc.date.issued 2020-10-27
dc.identifier.uri http://hdl.handle.net/10061/14441
dc.description.abstract Proline is a predominant amino acid in grape must, but it is poorly utilized by the yeast Saccharomyces cerevisiae in wine-making processes. This sometimes leads to a nitrogen deficiency during fermentation and proline accumulation in wine. Although the presence of other nitrogen sources under fermentation conditions is likely to interfere with proline utilization, the inhibitory mechanisms of proline utilization remain unclear. In this study, we examined the effect of arginine on proline utilization in S. cerevisiae. We first constructed a proline auxotrophic yeast strain and identified an inhibitory factor by observing the growth of cells when proline was present as a sole nitrogen source. Intriguingly, we found that arginine, and not ammonium ion, clearly inhibited the growth of proline auxotrophic cells. In addition, arginine prevented the proline consumption of wild-type and proline auxotrophic cells, indicating that arginine is an inhibitory factor of proline utilization in yeast. Next, quantitative polymerase chain reaction (PCR) analysis showed that arginine partially repressed the expression of genes involved in proline degradation and uptake. We then observed that arginine induced the endocytosis of the proline transporters Put4 and Gap1, whereas ammonium induced the endocytosis of only Gap1. Hence, our results may involve an important mechanism for arginine-mediated inhibition of proline utilization in yeast. The breeding of yeast that utilizes proline efficiently could be promising for the improvement of wine quality. ja_JP
dc.language.iso en ja_JP
dc.publisher Wiley ja_JP
dc.relation.isversionof https://onlinelibrary.wiley.com/doi/10.1002/yea.3504
dc.rights © 2020 John Wiley & Sons, Ltd. This is the peer reviewed version of the following article: [https://onlinelibrary.wiley.com/doi/10.1002/yea.3504], which has been published in final form at [https://doi.org/10.1002/yea.3504]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. 出版社許諾条件により、本文は2021年10月28日以降に公開 ja_JP
dc.subject arginine ja_JP
dc.subject endocytosis ja_JP
dc.subject nitrogen catabolite repression ja_JP
dc.subject proline utilization ja_JP
dc.subject Saccharomyces cerevisiae ja_JP
dc.title Inhibitory effect of arginine on proline utilization in Saccharomyces cerevisiae ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription ニシムラ, アキラ
dc.contributor.transcription タカギ, ヒロシ
dc.contributor.alternative 西村, 明
dc.contributor.alternative 高木, 博史
dc.textversion author ja_JP
dc.identifier.eissn 1097-0061
dc.identifier.jtitle Yeast ja_JP
dc.identifier.volume 37 ja_JP
dc.identifier.issue 9-10 ja_JP
dc.identifier.spage 531 ja_JP
dc.identifier.epage 540 ja_JP
dc.relation.doi 10.1002/yea.3504 ja_JP
dc.identifier.NAIST-ID 82040965 ja_JP
dc.identifier.NAIST-ID 73290561 ja_JP
dc.relation.pmid 32557770 ja_JP

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