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Non-aqueous fractionation revealed changing subcellular metabolite distribution during apple fruit development

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dc.contributor.author Beshir, Wasiye F. en
dc.contributor.author Tohge, Takayuki en
dc.contributor.author Watanabe, Mutsumi en
dc.contributor.author Hertog, Maarten L. A. T. M. en
dc.contributor.author Hoefgen, Rainer en
dc.contributor.author Fernie, Alisdair R. en
dc.contributor.author Nicolaï, Bart M. ja
dc.date.accessioned 2021-05-31T02:43:38Z en
dc.date.available 2021-05-31T02:43:38Z en
dc.date.issued 2019-08-11 en
dc.identifier.uri http://hdl.handle.net/10061/14280 en
dc.description.abstract In developing apple fruit, metabolic compartmentation is poorly understood due to the lack of experimental data. Distinguishing subcellular compartments in fruit using non-aqueous fractionation has been technically difficult due to the excess amount of sugars present in the different subcellular compartments limiting the resolution of the technique. The work described in this study represents the first attempt to apply non-aqueous fractionation to developing apple fruit, covering the major events occurring during fruit development (cell division, cell expansion, and maturation). Here we describe the non-aqueous fractionation method to study the subcellular compartmentation of metabolites during apple fruit development considering three main cellular compartments (cytosol, plastids, and vacuole). Evidence is presented that most of the sugars and organic acids were predominantly located in the vacuole, whereas some of the amino acids were distributed between the cytosol and the vacuole. The results showed a shift in the plastid marker from the lightest fractions in the early growth stage to the dense fractions in the later fruit growth stages. This implies that the accumulation of starch content with progressing fruit development substantially influenced the distribution of plastidial fragments within the non-aqueous density gradient applied. Results from this study provide substantial baseline information on assessing the subcellular compartmentation of metabolites in apple fruit in general and during fruit growth in particular. en
dc.language.iso en en
dc.publisher Springer Nature en
dc.relation.isreplacedby https://www.nature.com/articles/s41438-019-0178-7 en
dc.rights © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any mediumor format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. ja
dc.subject Metabolism en
dc.subject Metabolomics en
dc.subject Plant development en
dc.title Non-aqueous fractionation revealed changing subcellular metabolite distribution during apple fruit development en
dc.type.nii Journal Article en
dc.contributor.transcription トウゲ, タカユキ ja
dc.contributor.transcription ワタナベ, ムツミ ja
dc.contributor.alternative 峠, 隆之 ja
dc.contributor.alternative 渡辺, むつみ ja
dc.textversion none en
dc.identifier.eissn 2052-7276 en
dc.identifier.jtitle Horticulture Research en
dc.identifier.volume 6 en
dc.relation.doi 10.1038/s41438-019-0178-7 en
dc.identifier.artnum 98 en
dc.identifier.NAIST-ID 74653593 en
dc.identifier.NAIST-ID 74653866 en
dc.relation.pmid 31666959 en


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