DSpace Repository

Selective induction of targeted cell death and elimination by near-infrared femtosecond laser ablation

Show simple item record

dc.contributor.author Okano, Kazunori
dc.contributor.author Wang, Chung-Han
dc.contributor.author Hong, Zhen-Yi
dc.contributor.author Hosokawa, Yoichiroh
dc.contributor.author Liau, Ian
dc.date.accessioned 2021-02-15T08:52:00Z
dc.date.available 2021-02-15T08:52:00Z
dc.date.issued 2020-12
dc.identifier.uri http://hdl.handle.net/10061/14216
dc.description.abstract The techniques for inducing the death of specific cells in tissue has attracted attention as new methodologies for studying cell function and tissue regeneration. In this study, we show that a sequential process of targeted cell death and removal can be triggered by short-term exposure of near-infrared femtosecond laser pulses. Kinetic analysis of the intracellular accumulation of trypan blue and the assay of caspase activity revealed that femtosecond laser pulses induced immediate disturbance of plasma membrane integrity followed by apoptosis-like cell death. Yet, adjacent cells showed no sign of membrane damage and no increased caspase activity. The laser-exposed cells eventually detached from the substrate after a delay of >54 min while adjacent cells remained intact. On the base of in vitro experiments, we applied the same approach to ablate targeted single cardiac cells of a live zebrafish heart. The ability of inducing targeted cell death with femtosecond laser pulses should find broad applications that benefit from precise cellular manipulation at the level of single cells in vivo and in vitro. ja_JP
dc.language.iso en ja_JP
dc.publisher Elsevier ja_JP
dc.relation.isreplacedby https://www.sciencedirect.com/science/article/pii/S240558082030128X?via%3Dihub ja_JP
dc.rights © 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license ja_JP
dc.subject Cell death ja_JP
dc.subject Cell adhesion ja_JP
dc.subject Femtosecond laser ja_JP
dc.subject Ablation ja_JP
dc.subject Photo-apoptosis ja_JP
dc.title Selective induction of targeted cell death and elimination by near-infrared femtosecond laser ablation ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription ホソカワ, ヨウイチロウ
dc.contributor.alternative 細川, 陽一郎
dc.textversion none ja_JP
dc.identifier.jtitle Biochemistry and Biophysics Reports ja_JP
dc.identifier.volume 24 ja_JP
dc.relation.doi 10.1016/j.bbrep.2020.100818 ja_JP
dc.identifier.NAIST-ID 73295875 ja_JP


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account