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Establishment of a heart-on-a-chip microdevice based on human iPS cells for the evaluation of human heart tissue function

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dc.contributor.author Abulaiti, Mosha
dc.contributor.author Yalikun, Yaxiaer
dc.contributor.author Murata, Kozue
dc.contributor.author Sato, Asako
dc.contributor.author Sami, Mustafa M.
dc.contributor.author Sasaki, Yuko
dc.contributor.author Fujiwara, Yasue
dc.contributor.author Minatoya, Kenji
dc.contributor.author Shiba, Yuji
dc.contributor.author Tanaka, Yo
dc.contributor.author Masumoto, Hidetoshi
dc.date.accessioned 2021-02-15T08:46:51Z
dc.date.available 2021-02-15T08:46:51Z
dc.date.issued 2020-11-05
dc.identifier.uri http://hdl.handle.net/10061/14214
dc.description.abstract Human iPS cell (iPSC)-derived cardiomyocytes (CMs) hold promise for drug discovery for heart diseases and cardiac toxicity tests. To utilize human iPSC-derived CMs, the establishment of three-dimensional (3D) heart tissues from iPSC-derived CMs and other heart cells, and a sensitive bioassay system to depict physiological heart function are anticipated. We have developed a heart-on-a-chip microdevice (HMD) as a novel system consisting of dynamic culture-based 3D cardiac microtissues derived from human iPSCs and microelectromechanical system (MEMS)-based microfluidic chips. The HMDs could visualize the kinetics of cardiac microtissue pulsations by monitoring particle displacement, which enabled us to quantify the physiological parameters, including fluidic output, pressure, and force. The HMDs demonstrated a strong correlation between particle displacement and the frequency of external electrical stimulation. The transition patterns were validated by a previously reported versatile video-based system to evaluate contractile function. The patterns are also consistent with oscillations of intracellular calcium ion concentration of CMs, which is a fundamental biological component of CM contraction. The HMDs showed a pharmacological response to isoproterenol, a β-adrenoceptor agonist, that resulted in a strong correlation between beating rate and particle displacement. Thus, we have validated the basic performance of HMDs as a resource for human iPSC-based pharmacological investigations. ja_JP
dc.language.iso en ja_JP
dc.publisher nature ja_JP
dc.relation.isreplacedby https://www.nature.com/articles/s41598-020-76062-w ja_JP
dc.rights © 2020, The Author(s) This is an open access article distributed under the terms of the Creative Commons CC BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ja_JP
dc.title Establishment of a heart-on-a-chip microdevice based on human iPS cells for the evaluation of human heart tissue function ja_JP
dc.type.nii Journal Article ja_JP
dc.textversion none ja_JP
dc.identifier.eissn 2045-2322
dc.identifier.jtitle Scientific Reports ja_JP
dc.identifier.volume 10 ja_JP
dc.relation.doi 10.1038/s41598-020-76062-w ja_JP
dc.identifier.artnum 19201 (2020) ja_JP
dc.identifier.NAIST-ID 74654880 ja_JP

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