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Hydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low Reynolds numbers

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dc.contributor.author Zhang, Tianlong
dc.contributor.author Namoto, Misuzu
dc.contributor.author Okano, Kazunori
dc.contributor.author Akita, Eri
dc.contributor.author Teranishi, Norihiro
dc.contributor.author Tang, Tao
dc.contributor.author Anggraini, Dian
dc.contributor.author Hao, Yansheng
dc.contributor.author Tanaka, Yo
dc.contributor.author Inglis, David
dc.contributor.author Yalikun, Yaxiaer
dc.contributor.author Li, Ming
dc.contributor.author Hosokawa, Yoichiroh
dc.date.accessioned 2021-02-15T08:45:37Z
dc.date.available 2021-02-15T08:45:37Z
dc.date.issued 2021-01-18
dc.identifier.uri http://hdl.handle.net/10061/14213
dc.description.abstract Microfluidic focusing of particles (both synthetic and biological), which enables precise control over the positions of particles in a tightly focused stream, is a prerequisite step for the downstream processing, such as detection, trapping and separation. In this study, we propose a novel hydrodynamic focusing method by taking advantage of open v-shaped microstructures on a glass substrate engraved by femtosecond pulse (fs) laser. The fs laser engraved microstructures were capable of focusing polystyrene particles and live cells in rectangular microchannels at relatively low Reynolds numbers (Re). Numerical simulations were performed to explain the mechanisms of particle focusing and experiments were carried out to investigate the effects of groove depth, groove number and flow rate on the performance of the groove-embedded microchannel for particle focusing. We found out that 10-µm polystyrene particles are directed toward the channel center under the effects of the groove-induced secondary flows in low-Re flows, e.g. Re < 1. Moreover, we achieved continuous focusing of live cells with different sizes ranging from 10 to 15 µm, i.e. human T-cell lymphoma Jurkat cells, rat adrenal pheochromocytoma PC12 cells and dog kidney MDCK cells. The glass grooves fabricated by fs laser are expected to be integrated with on-chip detection components, such as contact imaging and fluorescence lifetime-resolved imaging, for various biological and biomedical applications, where particle focusing at a relatively low flow rate is desirable. ja_JP
dc.language.iso en ja_JP
dc.publisher Nature Research ja_JP
dc.relation.isreplacedby https://www.nature.com/articles/s41598-021-81190-y ja_JP
dc.rights © The Author(s) 2021 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creat iveco mmons .org/licen ses/by/4.0/. ja_JP
dc.title Hydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low Reynolds numbers ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription オカノ, カズノリ
dc.contributor.transcription アキタ, エリ
dc.contributor.transcription テラニシ, ノリヒロ
dc.contributor.transcription ホソカワ, ヨウイチロウ
dc.contributor.alternative 岡野, 和宣
dc.contributor.alternative 秋田, 絵理
dc.contributor.alternative 寺西, 教裕
dc.contributor.alternative 細川, 陽一郎
dc.textversion none ja_JP
dc.identifier.eissn 2045-2322
dc.identifier.jtitle Scientific Reports ja_JP
dc.identifier.volume 11 ja_JP
dc.relation.doi 10.1038/s41598-021-81190-y ja_JP
dc.identifier.artnum 1652 (2021) ja_JP
dc.identifier.NAIST-ID 86639556 ja_JP
dc.identifier.NAIST-ID 73295891 ja_JP
dc.identifier.NAIST-ID 74655135 ja_JP
dc.identifier.NAIST-ID 86637923 ja_JP
dc.identifier.NAIST-ID 87641486 ja_JP
dc.identifier.NAIST-ID 87641338 ja_JP
dc.identifier.NAIST-ID 86639507 ja_JP
dc.identifier.NAIST-ID 74654880 ja_JP
dc.identifier.NAIST-ID 73295875 ja_JP

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