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Large Gel Two-Dimensional Electrophoresis: Improving Recovery of

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dc.contributor.author Inagaki, Naoyuki
dc.contributor.author Katsuta, Kazuhiro
dc.date.accessioned 2020-12-28T00:52:56Z
dc.date.available 2020-12-28T00:52:56Z
dc.date.issued 2004
dc.identifier.issn 1570-1646
dc.identifier.uri http://hdl.handle.net/10061/14174
dc.description.abstract One of the goals of expression proteomics is to display and analyze all the proteins in a particular proteome. Cells are thought to comprise tens of thousands of proteins expressed in a dynamic range of 1-105 or 106. Low recovery of cellular proteome leads to a gross loss of important proteins. Thus, proteomics demands a powerful technology that separates complex mixture of proteins including low abundant ones. In the case of two-dimensional gel electrophoresis (2- DE), enlargement of the gel size appears a straightforward and effective strategy for improving the recovery of cellular proteins. Multiple narrow pH range immobilized pH gradients (nrIPGs) and long isoelectric focusing (IEF) gels afford improved separation of proteins in the first dimension according to isoelectric point. In addition, multiple long SDSPAGE gels of different polyacrylamide concentrations provide a tool to improve the resolution of the second dimension according to molecular weight. Recent data suggest that 2-DE with large gels can display more than 11,000 protein spots expressed in a 1-105 dynamic range in cells. ja_JP
dc.language.iso en ja_JP
dc.publisher Bentham Science Publishers ja_JP
dc.relation.isreplacedby https://www.eurekaselect.com/91128/article ja_JP
dc.rights ©2004 Bentham Science Publishers Ltd. ja_JP
dc.subject Proteome ja_JP
dc.subject Two-dimensional gel electrophoresis ja_JP
dc.subject Dynamic range ja_JP
dc.subject Low abundance ja_JP
dc.subject Resolution of separation ja_JP
dc.subject Recovery ja_JP
dc.title Large Gel Two-Dimensional Electrophoresis: Improving Recovery of ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription イナガキ, ナオユキ
dc.contributor.alternative 稲垣, 直之
dc.textversion author ja_JP
dc.identifier.jtitle Current Proteomics ja_JP
dc.identifier.volume 1 ja_JP
dc.identifier.issue 1 ja_JP
dc.identifier.spage 35 ja_JP
dc.identifier.epage 39 ja_JP
dc.relation.doi 10.2174/1570164043488289 ja_JP
dc.identifier.NAIST-ID 73290314 ja_JP

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