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Large Gel Two-Dimensional Electrophoresis: Improving Recovery of

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dc.contributor.author Inagaki, Naoyuki en
dc.contributor.author Katsuta, Kazuhiro en
dc.date.accessioned 2020-12-28T00:52:56Z en
dc.date.available 2020-12-28T00:52:56Z en
dc.date.issued 2004 en
dc.identifier.issn 1570-1646 en
dc.identifier.uri http://hdl.handle.net/10061/14174 en
dc.description.abstract One of the goals of expression proteomics is to display and analyze all the proteins in a particular proteome. Cells are thought to comprise tens of thousands of proteins expressed in a dynamic range of 1-105 or 106. Low recovery of cellular proteome leads to a gross loss of important proteins. Thus, proteomics demands a powerful technology that separates complex mixture of proteins including low abundant ones. In the case of two-dimensional gel electrophoresis (2- DE), enlargement of the gel size appears a straightforward and effective strategy for improving the recovery of cellular proteins. Multiple narrow pH range immobilized pH gradients (nrIPGs) and long isoelectric focusing (IEF) gels afford improved separation of proteins in the first dimension according to isoelectric point. In addition, multiple long SDSPAGE gels of different polyacrylamide concentrations provide a tool to improve the resolution of the second dimension according to molecular weight. Recent data suggest that 2-DE with large gels can display more than 11,000 protein spots expressed in a 1-105 dynamic range in cells. en
dc.language.iso en en
dc.publisher Bentham Science Publishers en
dc.relation.isreplacedby https://www.eurekaselect.com/91128/article en
dc.rights ©2004 Bentham Science Publishers Ltd. ja
dc.subject Proteome en
dc.subject Two-dimensional gel electrophoresis en
dc.subject Dynamic range en
dc.subject Low abundance en
dc.subject Resolution of separation en
dc.subject Recovery en
dc.title Large Gel Two-Dimensional Electrophoresis: Improving Recovery of en
dc.type.nii Journal Article en
dc.contributor.transcription イナガキ, ナオユキ ja
dc.contributor.alternative 稲垣, 直之 ja
dc.textversion author en
dc.identifier.jtitle Current Proteomics en
dc.identifier.volume 1 en
dc.identifier.issue 1 en
dc.identifier.spage 35 en
dc.identifier.epage 39 en
dc.relation.doi 10.2174/1570164043488289 en
dc.identifier.NAIST-ID 73290314 en

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