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ERK Activity Dynamics during Zebrafish Embryonic Development

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dc.contributor.author Wong, Kah-Loon
dc.contributor.author Akiyama, Ryutaro
dc.contributor.author Bessho, Yasumasa
dc.contributor.author Matsui, Takaaki
dc.date.accessioned 2020-11-24T02:43:05Z
dc.date.available 2020-11-24T02:43:05Z
dc.date.issued 2018-12-28
dc.identifier.uri http://hdl.handle.net/10061/14151
dc.description.abstract During vertebrate development, extracellular signal-regulated kinase (ERK) is activated by growth factors such as fibroblast growth factor (FGF), and it regulates the formation of tissues/organs including eyes, brains, somites, limbs, and inner ears. However, an experimental system to monitor ERK activity dynamics in the entire body of the vertebrate embryo is lacking. We recently studied ERK activity dynamics in the pre-somitic mesoderm of living zebrafish embryos injected with mRNAs encoding a Förster resonance energy transfer (FRET)-based ERK biosensor. In this study, transgenic zebrafish stably and ubiquitously expressing the ERK biosensor were generated to monitor ERK activity dynamics throughout embryonic development. The system allowed the identification of ERK activation domains in embryos from the late blastula to the late segmentation stage, consistent with immunostaining patterns obtained using anti-phosphorylated ERK antibody. A spatiotemporal map of ERK activity in the entire body during zebrafish embryogenesis was generated, and previously unidentified activation dynamics and ERK domains were identified. The proposed system is the first reported method to monitor ERK activity dynamics during vertebrate embryogenesis, providing insight into the role of ERK activity in normal and abnormal development in living vertebrate embryos. ja_JP
dc.language.iso en ja_JP
dc.publisher MDPI ja_JP
dc.relation.isreplacedby https://www.mdpi.com/1422-0067/20/1/109 ja_JP
dc.rights © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). ja_JP
dc.subject mitogen-activated protein kinase (MAPK) ja_JP
dc.subject biosensor ja_JP
dc.subject signal activity ja_JP
dc.subject vertebrate development ja_JP
dc.title ERK Activity Dynamics during Zebrafish Embryonic Development ja_JP
dc.type.nii Journal Article ja_JP
dc.textversion none ja_JP
dc.identifier.eissn 1422-0067
dc.identifier.jtitle International Journal of Molecular Sciences ja_JP
dc.relation.doi 10.3390/ijms20010109 ja_JP

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