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Noise-resistant developmental reproducibility in vertebrate somite formation

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dc.contributor.author Honda, Naoki
dc.contributor.author Akiyama, Ryutaro
dc.contributor.author Sari, Dini Wahyu Kartika
dc.contributor.author Ishii, Shin
dc.contributor.author Bessho, Yasumasa
dc.contributor.author Matsui, Takaaki
dc.date.accessioned 2020-11-24T02:39:14Z
dc.date.available 2020-11-24T02:39:14Z
dc.date.issued 2020-02-04
dc.identifier.uri http://hdl.handle.net/10061/14150
dc.description.abstract The reproducibility of embryonic development is remarkable, although molecular processes are intrinsically stochastic at the single-cell level. How the multicellular system resists the inevitable noise to acquire developmental reproducibility constitutes a fundamental question in developmental biology. Toward this end, we focused on vertebrate somitogenesis as a representative system, because somites are repeatedly reproduced within a single embryo whereas such reproducibility is lost in segmentation clock gene-deficient embryos. However, the effect of noise on developmental reproducibility has not been fully investigated, because of the technical difficulty in manipulating the noise intensity in experiments. In this study, we developed a computational model of ERK-mediated somitogenesis, in which bistable ERK activity is regulated by an FGF gradient, cell-cell communication, and the segmentation clock, subject to the intrinsic noise. The model simulation generated our previous in vivo observation that the ERK activity was distributed in a step-like gradient in the presomitic mesoderm, and its boundary was posteriorly shifted by the clock in a stepwise manner, leading to regular somite formation. Here, we showed that this somite regularity was robustly maintained against the noise. Removing the clock from the model predicted that the stepwise shift of the ERK activity occurs at irregular timing with irregular distance owing to the noise, resulting in somite size variation. This model prediction was recently confirmed by live imaging of ERK activity in zebrafish embryos. Through theoretical analysis, we presented a mechanism by which the clock reduces the inherent somite irregularity observed in clock-deficient embryos. Therefore, this study indicates a novel role of the segmentation clock in noise-resistant developmental reproducibility. ja_JP
dc.language.iso en ja_JP
dc.publisher Public Library of Science ja_JP
dc.relation.isreplacedby https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1006579 ja_JP
dc.rights © 2019 Naoki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ja_JP
dc.subject Somites ja_JP
dc.subject Embryos ja_JP
dc.subject ERK signaling cascade ja_JP
dc.subject Cell differentiation ja_JP
dc.subject Morphogenic segmentation ja_JP
dc.subject Zebrafish ja_JP
dc.subject Feedback regulation ja_JP
dc.subject Vertebrates ja_JP
dc.title Noise-resistant developmental reproducibility in vertebrate somite formation ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription アキヤマ, リュウタロウ
dc.contributor.transcription ベッショ, ヤスマサ
dc.contributor.transcription マツイ, タカアキ
dc.contributor.alternative 秋山, 隆太郎
dc.contributor.alternative 別所, 康全
dc.contributor.alternative 松井, 貴輝
dc.textversion none ja_JP
dc.identifier.eissn 1553-7358
dc.identifier.jtitle PLOS Computational Biology ja_JP
dc.relation.doi 10.1371/journal.pcbi.1006579 ja_JP
dc.identifier.NAIST-ID 82040809 ja_JP
dc.identifier.NAIST-ID 73290454 ja_JP
dc.identifier.NAIST-ID 73290470 ja_JP

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