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Time-lapse observation of stepwise regression of Erk activity in zebrafish presomitic mesoderm

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dc.contributor.author Sari, Dini Wahyu Kartika
dc.contributor.author Akiyama, Ryutaro
dc.contributor.author Honda, Naoki
dc.contributor.author Ishijima, Hannosuke
dc.contributor.author Bessho, Yasumasa
dc.contributor.author Matsui, Takaaki
dc.date.accessioned 2020-11-24T02:19:53Z
dc.date.available 2020-11-24T02:19:53Z
dc.date.issued 2018-03-12
dc.identifier.uri http://hdl.handle.net/10061/14149
dc.description.abstract During somite segmentation, clock genes oscillate within the posterior presomitic mesoderm (PSM). The temporal information ties up with the posteriorly moving FGF gradient, leading to the formation of a presumptive somite within the PSM. We previously investigated Erk activity downstream of FGF signaling by collecting stained zebrafish embryos, and discovered that the steep gradient of Erk activity was generated in the PSM, and the Erk activity border regularly shifted in a stepwise manner. However, since these interpretations come from static analyses, we needed to firmly confirm them by applying an analysis that has higher spatiotemporal resolutions. Here we developed a live imaging system for Erk activity in zebrafish embryos, using a Förster resonance energy transfer (FRET)-based Erk biosensor. With this system, we firmly showed that Erk activity exhibits stepwise regression within the PSM. Although our static analyses could not detect the stepwise pattern of Erk activity in clock-deficient embryos, our system revealed that, in clock-deficient embryos, the stepwise regression of Erk activity occurs at an irregular timing, eventually leading to formation of irregularly-sized somites. Therefore, our system overcame the limitation of static analyses and revealed that clock-dependent spatiotemporal regulation of Erk is required for proper somitogenesis in zebrafish. ja_JP
dc.language.iso en ja_JP
dc.publisher Nature Research ja_JP
dc.relation.isreplacedby https://www.nature.com/articles/s41598-018-22619-9 ja_JP
dc.rights © The Author(s) 2018 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. ja_JP
dc.title Time-lapse observation of stepwise regression of Erk activity in zebrafish presomitic mesoderm ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription アキヤマ, リュウタロウ
dc.contributor.transcription ベッショ, ヤスマサ
dc.contributor.transcription マツイ, タカアキ
dc.contributor.alternative 秋山, 隆太郎
dc.contributor.alternative 別所, 康全
dc.contributor.alternative 松井, 貴輝
dc.textversion none ja_JP
dc.identifier.eissn 2045-2322
dc.identifier.jtitle Scientific Reports ja_JP
dc.identifier.volume 8 ja_JP
dc.relation.doi 10.1038/s41598-018-22619-9 ja_JP
dc.identifier.artnum 4335 (2018) ja_JP
dc.identifier.NAIST-ID 82040809 ja_JP
dc.identifier.NAIST-ID 73290454 ja_JP
dc.identifier.NAIST-ID 73290470 ja_JP

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