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Photoinjection of fluorescent nanoparticles into intact plant cells using femtosecond laser amplifier

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dc.contributor.author Rukmana, Taufiq Indra en
dc.contributor.author Moran, Gabriela en
dc.contributor.author Méallet-Renault, Rachel ja
dc.contributor.author Clavier, Gilles en
dc.contributor.author Kunieda, Tadashi en
dc.contributor.author Ohtani, Misato en
dc.contributor.author Demura, Taku en
dc.contributor.author Yasukuni, Ryohei en
dc.contributor.author Hosokawa, Yoichiroh en
dc.date.accessioned 2020-10-28T05:22:58Z en
dc.date.available 2020-10-28T05:22:58Z en
dc.date.issued 2020-06-15 en
dc.identifier.uri http://hdl.handle.net/10061/14131 en
dc.description.abstract The introduction of nanoparticles to intact plant cells is promising as a transporting technique of a wide range of functional molecules. Among various molecular delivery methods, femtosecond laser photoinjection possesses target selectivity at a single cell level and is potentially applicable for many types of materials. However, for plant cells, the vacuoles’ turgor pressure and the thick cell wall limit the application of photoinjection to only small objects. In this work, we overcome these limitations by employing a single pulse irradiation from a femtosecond laser amplifier. After laser irradiation on intact tobacco BY-2 cells, 80 nm fluorescent nanoparticles dispersed in a cell culture medium were successfully injected into their cytoplasm. This breakthrough would lead to a vast utilization of nanoparticles containing functional molecules for single cell manipulation in plant physiological study and genetic engineering. Such an injection was observed even when the laser pulse was focused neither on the cell wall nor on the cell membrane, but beside the cells. With these results, we suggest pore formation on the cell membrane by instantaneous deformation induced by an intense femtosecond laser pulse as an injection mechanism of nanoparticles. Reported photomechanical effects of the amplified femtosecond laser on the permeability of the biological membrane would offer new perspectives in biophotonics. ja
dc.language.iso en en
dc.relation.isreplacedby https://aip.scitation.org/doi/10.1063/5.0001687 en
dc.rights © 2020 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). https://doi.org/10.1063/5.0001687 ja
dc.title Photoinjection of fluorescent nanoparticles into intact plant cells using femtosecond laser amplifier en
dc.type.nii Journal Article en
dc.contributor.transcription クニエダ, タダシ ja
dc.contributor.transcription オオタニ, ミサト ja
dc.contributor.transcription デムラ, タク ja
dc.contributor.transcription ヤスクニ, リョウヘイ ja
dc.contributor.transcription ホソカワ, ヨウイチロウ ja
dc.contributor.alternative 國枝, 正 ja
dc.contributor.alternative 大谷, 美沙都 ja
dc.contributor.alternative 出村, 拓 ja
dc.contributor.alternative 安國, 良平 ja
dc.contributor.alternative 細川, 陽一郎 ja
dc.textversion none en
dc.identifier.eissn 1941-420X en
dc.identifier.jtitle APL Photonics en
dc.identifier.volume 5 en
dc.identifier.issue 6 en
dc.relation.doi 10.1063/5.0001687 en
dc.identifier.NAIST-ID 86630142 en
dc.identifier.NAIST-ID 74654435 en
dc.identifier.NAIST-ID 74650995 en
dc.identifier.NAIST-ID 73290702 en
dc.identifier.NAIST-ID 74653262 en
dc.identifier.NAIST-ID 73295875 en

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