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Mitochondrial cysteinyl-tRNA synthetase is expressed via alternative transcriptional initiation regulated by energy metabolism in yeast cells

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dc.contributor.author Nishimura, Akira en
dc.contributor.author Nasuno, Ryo en
dc.contributor.author Yoshikawa, Yuki en
dc.contributor.author Jung, Minkyung en
dc.contributor.author Ida, Tomoaki en
dc.contributor.author Matsunaga, Tetsuro en
dc.contributor.author Morita, Masanobu en
dc.contributor.author Takagi, Hiroshi en
dc.contributor.author Motohashi, Hozumi en
dc.contributor.author Akaike, Takaaki en
dc.date.accessioned 2020-10-19T06:09:59Z en
dc.date.available 2020-10-19T06:09:59Z en
dc.date.issued 2019-09-13 en
dc.identifier.uri http://hdl.handle.net/10061/14113 en
dc.description.abstract Eukaryotes typically utilize two distinct aminoacyl-tRNA synthetase isoforms, one for cytosolic and one for mitochondrialprotein synthesis. However, the genome of budding yeast (Saccharomyces cerevisiae) contains only one cysteinyl-tRNA synthetase gene (YNL247W, also known as CRS1). In this study, we report that CRS1 encodes both cytosolic and mitochondrial isoforms. The 5′ complementary DNA end method and GFP reporter gene analyses indicatedthat yeast CRS1 expression yields two classes of mRNAs through alternative transcription starts: a long mRNA containing a mitochondrial targetingsequence and a short mRNA lacking this targeting sequence. We found that the mitochondrial Crs1 is the product of translationfrom the first initiation AUG codon on the long mRNA, whereas the cytosolic Crs1 is produced from the second in-frame AUGcodon on the short mRNA. Genetic analysis and a ChIP assay revealed that the transcription factor heme activator protein (Hap)complex, which is involved in mitochondrial biogenesis, determines the transcription start sites of the CRS1 gene. We also noted that Hap complex–dependent initiation is regulated according to the needs of mitochondrial energy production.The results of our study indicate energy-dependent initiation of alternative transcription of CRS1 that results in production of two Crs1 isoforms, a finding that suggests Crs1's potential involvement in mitochondrial energymetabolism in yeast. ja
dc.language.iso en en
dc.publisher American Society for Biochemistry and Molecular Biology en
dc.relation.isreplacedby https://www.jbc.org/content/294/37/13781.full en
dc.rights © 2019 Nishimura et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. ja
dc.subject Hap complex en
dc.subject alternative transcription en
dc.subject aminoacyl-tRNA synthetase en
dc.subject cysteinyl-tRNA synthetase en
dc.subject energy metabolism en
dc.subject gene regulation en
dc.subject mitochondrial bioenergetics en
dc.subject transcription en
dc.subject transcriptional start site en
dc.subject yeast en
dc.title Mitochondrial cysteinyl-tRNA synthetase is expressed via alternative transcriptional initiation regulated by energy metabolism in yeast cells en
dc.type.nii Journal Article en
dc.contributor.transcription ニシムラ, アキラ ja
dc.contributor.transcription ナスノ, リョウ ja
dc.contributor.transcription ヨシカワ, ユウキ ja
dc.contributor.transcription タカギ, ヒロシ ja
dc.contributor.alternative 西村, 明 ja
dc.contributor.alternative 那須野, 亮 ja
dc.contributor.alternative 吉川, 雄樹 ja
dc.contributor.alternative 高木, 博史 ja
dc.textversion none en
dc.identifier.eissn 1083-351X en
dc.identifier.jtitle Journal of Biological Chemistry en
dc.relation.doi 10.1074/jbc.RA119.009203 en
dc.identifier.NAIST-ID 82040965 en
dc.identifier.NAIST-ID 82045303 en
dc.identifier.NAIST-ID 83515130 en
dc.identifier.NAIST-ID 73290561 en

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