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Involvement of I-BAR protein IRSp53 in tumor cell growth via extracellular microvesicle secretion

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dc.contributor.author Hu, Hooi Ting
dc.contributor.author Sasakura, Naoto
dc.contributor.author Matsubara, Daisuke
dc.contributor.author Furusawa, Naoko
dc.contributor.author Mukai, Masahiro
dc.contributor.author Kitamura, Narufumi
dc.contributor.author Obayashi, Takeshi
dc.contributor.author Nishimura, Tamako
dc.contributor.author Oono-Yakura, Kayoko
dc.contributor.author Funato, Yosuke
dc.contributor.author Okamura, Yasunobu
dc.contributor.author Tarao, Kento
dc.contributor.author Nakano, Yasushi
dc.contributor.author Murakami, Yoshinori
dc.contributor.author Kinoshita, Kengo
dc.contributor.author Takahashi, Chiaki
dc.contributor.author Miki, Hiroaki
dc.contributor.author Gonda, Kohsuke
dc.contributor.author Scita, Giorgio
dc.contributor.author Hanawa-Suetsugu, Kyoko
dc.contributor.author Suetsugu, Shiro
dc.date.accessioned 2020-09-23T10:29:04Z
dc.date.available 2020-09-23T10:29:04Z
dc.date.issued 2020-04-20
dc.identifier.uri http://hdl.handle.net/10061/14062
dc.description.abstract Cellular protrusions mediated by the membrane-deforming I-BAR domain protein IRSp53 are involved in cell migration, including metastasis. However, the role of IRSp53 in cell proliferation remains unclear. Here, we examined the role of IRSp53 in cell proliferation and found that it acts through secretion. Coculture of gingiva squamous carcinoma Ca9-22 cells and their IRSp53-knockout cells restored proliferation to parental Ca9-22 cell levels, suggesting possible secretion dependent on IRSp53. Notably, the amounts of microvesicle fraction proteins that were secreted into the culture medium were reduced in the IRSp53-knockout cells. The IRSp53-knockout cells exhibited decreased phosphorylation of mitogen-activated protein kinase, suggesting the decrease in the proliferation signals. The phosphorylation was restored by the addition of the microvesicles. In mice xenograft Ca9-22 cells, IRSp53-containing particles were secreted around the xenograft, indicating that IRSp53-dependent secretion occurs in vivo. In a tumor mice model, IRSp53 deficiency elongated lifespan. In some human cancers, the higher levels of IRSp53 mRNA expression was found to be correlated with shorter survival years. Therefore, IRSp53 is involved in tumor progression and secretion for cellular proliferation. ja_JP
dc.language.iso en ja_JP
dc.publisher Cold Spring Harbor Laboratory ja_JP
dc.relation.isreplacedby https://www.biorxiv.org/content/10.1101/2020.04.20.050492v1 ja_JP
dc.rights The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. ja_JP
dc.title Involvement of I-BAR protein IRSp53 in tumor cell growth via extracellular microvesicle secretion ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription ニシムラ, タマコ
dc.contributor.transcription ヤクラ, カヨコ
dc.contributor.transcription スエツグ, シロウ
dc.contributor.alternative 西村, 珠子
dc.contributor.alternative 矢倉, 加代子
dc.contributor.alternative 末次, 志郎
dc.textversion none ja_JP
dc.identifier.jtitle bioRxiv ja_JP
dc.relation.doi 10.1101/2020.04.20.050492 ja_JP
dc.identifier.NAIST-ID 86634185 ja_JP
dc.identifier.NAIST-ID 74654088 ja_JP
dc.identifier.NAIST-ID 74651753 ja_JP
dc.identifier.NAIST-ID 74650532 ja_JP

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