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Regulation of caveolae through cholesterol-depletion dependent tubulation by PACSIN2/Syndapin II

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dc.contributor.author Amir, Aini Gusmira
dc.contributor.author Takemura, Kazuhiro
dc.contributor.author Hanawa-Suetsugu, Kyoko
dc.contributor.author Oono-Yakura, Kayoko
dc.contributor.author Yasuhara, Kazuma
dc.contributor.author Kitao, Akio
dc.contributor.author Suetsugu, Shiro
dc.date.accessioned 2020-09-23T10:20:36Z
dc.date.available 2020-09-23T10:20:36Z
dc.date.issued 2020-03-25
dc.identifier.uri http://hdl.handle.net/10061/14061
dc.description.abstract The membrane shaping ability of PACSIN2 via its FCH-BAR (F-BAR) domain has been shown to be essential for caveolar morphogenesis, presumably through the shaping of the caveolar neck. Caveolar membrane contains abundant levels of cholesterol. However, the role of cholesterol in PACSIN2-mediated membrane deformation remains unclear. We show that the binding of PACSIN2 to the membrane could be negatively regulated by the amount of cholesterol in the membrane. We prepared a reconstituted membrane based on the lipid composition of caveolae. The reconstituted membrane with cholesterol had a weaker affinity to the F-BAR domain of PACSIN2 than the membrane without cholesterol, presumably due to a decrease in electrostatic charge density. Consistently, the acute depletion of cholesterol from the plasma membrane resulted in the appearance of PACSIN2-localized tubules with caveolin-1 at their tips, suggesting that the presence of cholesterol inhibited the prominent membrane tubulation by PACSIN2. The tubules induced by PACSIN2 were suggested to be an intermediate of caveolae endocytosis. Consistently, the removal of caveolae from the plasma membrane upon cholesterol depletion was diminished in the cells deficient in PACSIN2. These data suggested that PACSIN2 mediated the caveolae internalization dependently on the amount of cholesterol at the plasma membrane, providing a possible mechanism for the cholesterol-dependent regulation of caveolae. ja_JP
dc.language.iso en ja_JP
dc.publisher Cold Spring Harbor Laboratory ja_JP
dc.relation.isreplacedby https://www.biorxiv.org/content/10.1101/2020.03.25.008854v1 ja_JP
dc.rights The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. ja_JP
dc.title Regulation of caveolae through cholesterol-depletion dependent tubulation by PACSIN2/Syndapin II ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription ヤクラ, カヨコ
dc.contributor.transcription ヤスハラ, カズマ
dc.contributor.transcription スエツグ, シロウ
dc.contributor.alternative 矢倉, 加代子
dc.contributor.alternative 安原, 主馬
dc.contributor.alternative 末次, 志郎
dc.textversion none ja_JP
dc.identifier.jtitle bioRxiv ja_JP
dc.relation.doi 10.1101/2020.03.25.008854 ja_JP
dc.identifier.NAIST-ID 74651753 ja_JP
dc.identifier.NAIST-ID 73293482 ja_JP
dc.identifier.NAIST-ID 74650532 ja_JP

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