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Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake

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dc.contributor.author Tanaka, Yoshiki
dc.contributor.author Yoshikaie, Kunihito
dc.contributor.author Takeuchi, Azusa
dc.contributor.author Ichikawa, Muneyoshi
dc.contributor.author Mori, Tomoyuki
dc.contributor.author Uchino, Sayaka
dc.contributor.author Sugano, Yasunori
dc.contributor.author Hakoshima, Toshio
dc.contributor.author Takagi, Hiroshi
dc.contributor.author Nonaka, Gen
dc.contributor.author Tsukazaki, Tomoya
dc.date.accessioned 2020-09-07T07:22:48Z
dc.date.available 2020-09-07T07:22:48Z
dc.date.issued 2020-08-26
dc.identifier.uri http://hdl.handle.net/10061/14046
dc.description.abstract We have demonstrated that a bacterial membrane protein, YeeE, mediates thiosulfate uptake. Thiosulfate is used for cysteine synthesis in bacteria as an inorganic sulfur source in the global biological sulfur cycle. The crystal structure of YeeE at 2.5-Å resolution reveals an unprecedented hourglass-like architecture with thiosulfate in the positively charged outer concave side. YeeE is composed of loops and 13 helices including 9 transmembrane α helices, most of which show an intramolecular pseudo 222 symmetry. Four characteristic loops are buried toward the center of YeeE and form its central region surrounded by the nine helices. Additional electron density maps and successive molecular dynamics simulations imply that thiosulfate can remain temporally at several positions in the proposed pathway. We propose a plausible mechanism of thiosulfate uptake via three important conserved cysteine residues of the loops along the pathway. ja_JP
dc.language.iso en ja_JP
dc.publisher American Association for the Advancement of Science ja_JP
dc.relation.isreplacedby https://advances.sciencemag.org/content/6/35/eaba7637 ja_JP
dc.rights Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). ja_JP
dc.title Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription タナカ, ヨシキ
dc.contributor.transcription ヨシカイエ, クニヒト
dc.contributor.transcription タケウチ, アズサ
dc.contributor.transcription モリ, トモユキ
dc.contributor.transcription ハコシマ, トシオ
dc.contributor.transcription タカギ, ヒロシ
dc.contributor.transcription ツカザキ, トモヤ
dc.contributor.alternative 田中, 良樹
dc.contributor.alternative 吉海江, 国仁
dc.contributor.alternative 竹内, 梓
dc.contributor.alternative 森, 智行
dc.contributor.alternative 箱嶋, 敏雄
dc.contributor.alternative 高木, 博史
dc.contributor.alternative 塚崎, 智也
dc.textversion none ja_JP
dc.identifier.eissn 2375-2548
dc.identifier.jtitle Science Advances ja_JP
dc.identifier.volume 6 ja_JP
dc.identifier.issue 35 ja_JP
dc.relation.doi 10.1126/sciadv.aba7637 ja_JP
dc.identifier.NAIST-ID 74650169 ja_JP
dc.identifier.NAIST-ID 74651480 ja_JP
dc.identifier.NAIST-ID 86637691 ja_JP
dc.identifier.NAIST-ID 73295396 ja_JP
dc.identifier.NAIST-ID 73292344 ja_JP
dc.identifier.NAIST-ID 73290561 ja_JP
dc.identifier.NAIST-ID 73299737 ja_JP

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