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Hereditary Pancreatitis Model by Blastocyst Complementation in Mouse

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dc.contributor.author Asai, Ayumu
dc.contributor.author Konno, Masamitsu
dc.contributor.author Kawamoto, Koichi
dc.contributor.author Isotani, Ayako
dc.contributor.author Mori, Masaki
dc.contributor.author Eguchi, Hidetoshi
dc.contributor.author Doki, Yuichiro
dc.contributor.author Arai, Takahiro
dc.contributor.author Ishii, Hideshi
dc.date.accessioned 2020-08-25T02:12:44Z
dc.date.available 2020-08-25T02:12:44Z
dc.date.issued 2020-06-02
dc.identifier.uri http://hdl.handle.net/10061/14029
dc.description.abstract The application of pluripotent stem cells is expected to contribute to the elucidation of unknown mechanism of human diseases. However, in vitro induction of organ-specific cells, such as pancreas and liver, is still difficult and the reproduction of their disorders in a model has been unfeasible. To study the mechanism of human hereditary pancreatitis (HP), we here performed the blastocyst complementation (BC) method. In the BC method, mouse embryonic stem (ES) cells harboring CRISPR/CAS9-mediated mutations in the Prss1 gene were injected into blastocysts with deficient Pdx1 gene, which is a critical transcription factor in the development of pancreas. The results showed that trypsin was activated extremely in Prss1-mutant mice. This implied that the mouse phenotype mimics that of human HP and that the BC method was useful for the reproduction and study of pancreatic disorders. The present study opens the possibility of investigating uncharacterized human diseases by utilizing the BC method. ja_JP
dc.language.iso en ja_JP
dc.publisher Impact Journals ja_JP
dc.relation.isreplacedby https://www.oncotarget.com/article/27595/text/ ja_JP
dc.rights Asai et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and sourceare credited. ja_JP
dc.subject blastocyst complementation; ja_JP
dc.subject hereditary pancreatitis ja_JP
dc.subject hereditary pancreatitis ja_JP
dc.subject PRSS1 ja_JP
dc.title Hereditary Pancreatitis Model by Blastocyst Complementation in Mouse ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription イソタニ, アヤコ
dc.contributor.alternative 磯谷, 綾子
dc.textversion none ja_JP
dc.identifier.eissn 1949-2553
dc.identifier.jtitle Oncotarget ja_JP
dc.identifier.volume 11 ja_JP
dc.identifier.issue 22 ja_JP
dc.identifier.spage 2061 ja_JP
dc.identifier.epage 2073 ja_JP
dc.relation.doi 10.18632/oncotarget.27595 ja_JP
dc.identifier.NAIST-ID 74653114 ja_JP
dc.relation.pmid 32547704 ja_JP

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