DSpace Repository

Raman image-activated cell sorting

Show simple item record

dc.contributor.author Nitta, Nao en
dc.contributor.author Iino, Takanori en
dc.contributor.author Isozaki, Akihiro en
dc.contributor.author Yamagishi, Mai en
dc.contributor.author Kitahama, Yasutaka en
dc.contributor.author Sakuma, Shinya en
dc.contributor.author Suzuki, Yuta en
dc.contributor.author Tezuka, Hiroshi en
dc.contributor.author Oikawa, Minoru en
dc.contributor.author Arai, Fumihito en
dc.contributor.author Asai, Takuya en
dc.contributor.author Deng, Dinghuan en
dc.contributor.author Fukuzawa, Hideya en
dc.contributor.author Hase, Misa en
dc.contributor.author Hasunuma, Tomohisa en
dc.contributor.author Hayakawa, Takeshi en
dc.contributor.author Hiraki, Kei en
dc.contributor.author Hiramatsu, Kotaro en
dc.contributor.author Hoshino, Yu en
dc.contributor.author Inaba, Mary en
dc.contributor.author Inoue, Yuki en
dc.contributor.author Ito, Takuro en
dc.contributor.author Kajikawa, Masataka en
dc.contributor.author Karakawa, Hiroshi en
dc.contributor.author Kasai, Yusuke en
dc.contributor.author Kato, Yuichi en
dc.contributor.author Kobayashi, Hirofumi en
dc.contributor.author Lei, Cheng en
dc.contributor.author Matsusaka, Satoshi en
dc.contributor.author Mikami, Hideharu en
dc.contributor.author Nakagawa, Atsuhiro en
dc.contributor.author Numata, Keiji en
dc.contributor.author Ota, Tadataka en
dc.contributor.author Sekiya, Takeichiro en
dc.contributor.author Shiba, Kiyotaka en
dc.contributor.author Shirasaki, Yoshitaka en
dc.contributor.author Suzuki, Nobutake en
dc.contributor.author Tanaka, Shunji en
dc.contributor.author Ueno, Shunnosuke en
dc.contributor.author Watarai, Hiroshi en
dc.contributor.author Yamano, Takashi en
dc.contributor.author Yazawa, Masayuki en
dc.contributor.author Yonamine, Yusuke en
dc.contributor.author Di Carlo, Dino en
dc.contributor.author Hosokawa, Yoichiroh en
dc.contributor.author Uemura, Sotaro en
dc.contributor.author Sugimura, Takeaki en
dc.contributor.author Ozeki, Yasuyuki en
dc.contributor.author Goda, Keisuke en
dc.date.accessioned 2020-08-03T09:17:02Z en
dc.date.available 2020-08-03T09:17:02Z en
dc.date.issued 2020-07-10 en
dc.identifier.issn 2041-1723 en
dc.identifier.uri http://hdl.handle.net/10061/14010 en
dc.description.abstract The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demonstrate Raman image-activated cell sorting by directly probing chemically specific intracellular molecular vibrations via ultrafast multicolor stimulated Raman scattering (SRS) microscopy for cellular phenotyping. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to ~100 events per second without the need for fluorescent labeling. To show the broad utility of the technology, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies. en
dc.language.iso en en
dc.publisher Springer Nature en
dc.relation.isreplacedby https://www.nature.com/articles/s41467-020-17285-3 en
dc.rights © The Author(s) 2020 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. ja
dc.subject High-throughput screening en
dc.subject Imaging en
dc.subject Isolation, separation and purification en
dc.subject Lab-on-a-chip en
dc.subject Raman spectroscopy en
dc.title Raman image-activated cell sorting en
dc.type.nii Journal Article en
dc.contributor.transcription ホソカワ, ヨウイチロウ ja
dc.contributor.alternative 細川, 陽一郎 ja
dc.textversion none en
dc.identifier.jtitle Nature Communications en
dc.identifier.volume 11 en
dc.relation.doi 10.1038/s41467-020-17285-3 en
dc.identifier.artnum 3452 (2020) en
dc.identifier.NAIST-ID 73295875 en
dc.relation.pmid 32651381 en


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account