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Detection system of the intracellular nitric oxide in yeast by HPLC with a fluorescence detector

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dc.contributor.author Nasuno, Ryo
dc.contributor.author Shino, Seiya
dc.contributor.author Yoshikawa, Yuki
dc.contributor.author Yoshioka, Natsuko
dc.contributor.author Sato, Yuichi
dc.contributor.author Kamiya, Kohei
dc.contributor.author Hiroshi, Takagi
dc.date.accessioned 2020-05-28T05:26:55Z
dc.date.available 2020-05-28T05:26:55Z
dc.date.issued 2020-06-01
dc.identifier.issn 0003-2697
dc.identifier.uri http://hdl.handle.net/10061/13986
dc.description.abstract Nitric oxide (NO) is an important signaling molecule involved in various biological phenomena in many organisms. The physiological functions and metabolism of NO in yeast, a unicellular microorganism, are still unknown, mainly because it is difficult to analyze the intracellular NO levels accurately. Here, we developed a new method of more accurately measuring NO content in yeast cells with the detection limit of 6 nM, by treating the cells with an NO-specific fluorescence probe followed by high-performance liquid chromatography with fluorescence detection (HPLC/FLD). This approach successfully detected and quantified the NO content inside yeast cells treated with an NO donor. Moreover, the HPLC/FLD analysis indicates that the fluorescence induced under some environmental stress conditions, such as ethanol, vanillin, and heat-shock, was not derived from NO. The HPLC/FLD method developed in this study provides a new strategy for measuring the intracellular NO concentration with higher accuracy. ja_JP
dc.language.iso en ja_JP
dc.publisher Elsevier ja_JP
dc.rights © 2020 Elsevier Inc. All rights reserved. ja_JP
dc.subject Nitric oxide ja_JP
dc.subject Fluorescence probe ja_JP
dc.subject Methodology ja_JP
dc.subject Yeast ja_JP
dc.subject Environmental stress ja_JP
dc.title Detection system of the intracellular nitric oxide in yeast by HPLC with a fluorescence detector ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription ナスノ, リョウ
dc.contributor.transcription シノ, セイヤ
dc.contributor.transcription ヨシカワ, ユウキ
dc.contributor.transcription ヨシオカ, ナツコ
dc.contributor.transcription サトウ, ユウイチ
dc.contributor.transcription カミヤ, コウヘイ
dc.contributor.transcription タカギ, ヒロシ
dc.contributor.alternative 那須野, 亮
dc.contributor.alternative 示野, 誠也
dc.contributor.alternative 吉川, 雄樹
dc.contributor.alternative 吉岡, 奈津子
dc.contributor.alternative 佐藤, 裕一
dc.contributor.alternative 神谷, 紘平
dc.contributor.alternative 高木, 博史
dc.textversion author ja_JP
dc.identifier.jtitle Analytical Biochemistry ja_JP
dc.identifier.volume 598 ja_JP
dc.relation.doi 10.1016/j.ab.2020.113707 ja_JP
dc.identifier.NAIST-ID 82045303 ja_JP
dc.identifier.NAIST-ID 85627891 ja_JP
dc.identifier.NAIST-ID 83515130 ja_JP
dc.identifier.NAIST-ID 86633740 ja_JP
dc.identifier.NAIST-ID 86631934 ja_JP
dc.identifier.NAIST-ID 86631454 ja_JP
dc.identifier.NAIST-ID 73290561 ja_JP


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