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Enzyme-Assisted Photoinjection of Megadalton Molecules into Intact Plant Cells Using Femtosecond Laser Amplifier

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dc.contributor.author Taufiq, Indra Rukmana en
dc.contributor.author Gabriela, Moran en
dc.contributor.author Rachel, Méallet-Renault en
dc.contributor.author Ohtani, Misato en
dc.contributor.author Demura, Taku en
dc.contributor.author Yasukuni, Ryohei en
dc.contributor.author Hosokawa, Yoichiroh en
dc.date.accessioned 2020-05-21T02:28:48Z
dc.date.available 2020-05-21T02:28:48Z
dc.date.issued 2019-11-26
dc.identifier.uri http://hdl.handle.net/10061/13983
dc.description.abstract Femtosecond laser photoporation has become a popular method to deliver various kinds of molecules such as genes, proteins, and fluorescent dyes into single mammalian cells. However, this method is not easily applied to plant cells because their cell wall and turgor pressure prevent the delivery, especially for larger molecules than the mesh size of the cell wall. This work is the first demonstration of the efficient photoinjection of megadalton molecules into a cytoplasm of an intact single plant cell by employing a femtosecond laser amplifier under moderate enzyme treatment conditions. The intense femtosecond laser pulse effectively formed a pore on the cell wall and membrane of Tobacco BY-2, and 2 MDa dextran molecules were introduced through the pore. Along with the pore formation, induced mechanical tensile stresses on BY-2 cells were considered to increase permeability of the cell membrane and enhance the uptake of large molecules. Moreover, the moderate enzyme treatment partially degraded the cell wall thereby facilitating the increase of the molecular introduction efficiency. en
dc.language.iso en
dc.publisher nature en
dc.relation.isreplacedby https://www.nature.com/articles/s41598-019-54124-y.pdf
dc.rights © The Author(s) 2019 en
dc.subject Gene delivery en
dc.subject Optical techniques en
dc.subject Plant biotechnology en
dc.title Enzyme-Assisted Photoinjection of Megadalton Molecules into Intact Plant Cells Using Femtosecond Laser Amplifier en
dc.type.nii Journal Article
dc.contributor.transcription オオタニ, ミサト ja-Kana
dc.contributor.transcription デムラ, タク ja-Kana
dc.contributor.transcription ヤスクニ, リョウヘイ ja-Kana
dc.contributor.transcription ホソカワ, ヨウイチロウ ja-Kana
dc.contributor.alternative 大谷, 美沙都 ja
dc.contributor.alternative 出村, 拓 ja
dc.contributor.alternative 安國, 良平 ja
dc.contributor.alternative 細川, 陽一郎 ja
dc.textversion none
dc.identifier.jtitle Scientific Reports en
dc.identifier.volume 9
dc.relation.doi 10.1038/s41598-019-54124-y
dc.identifier.artnum 17530
dc.identifier.NAIST-ID 86630142
dc.identifier.NAIST-ID 74650995
dc.identifier.NAIST-ID 73290702
dc.identifier.NAIST-ID 74653262
dc.identifier.NAIST-ID 73295875


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