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Live cell imaging of meiosis in Arabidopsis thaliana

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dc.contributor.author Maria A, Prusicki en
dc.contributor.author Emma M, Keizer en
dc.contributor.author Rik P, van Rosmalen en
dc.contributor.author Komaki, Shinichiro en
dc.contributor.author Seifert, Felix en
dc.contributor.author Katja, Müller ja
dc.contributor.author Erik, Wijnker en
dc.contributor.author Christian, Fleck en
dc.contributor.author Arp, Schnittger en
dc.date.accessioned 2019-12-11T08:34:58Z en
dc.date.available 2019-12-11T08:34:58Z en
dc.date.issued 2019-05-20 en
dc.identifier.uri http://hdl.handle.net/10061/13952 en
dc.description.abstract To follow the dynamics of meiosis in the model plant Arabidopsis, we have established a live cell imaging setup to observe male meiocytes. Our method is based on the concomitant visualization of microtubules (MTs) and a meiotic cohesin subunit that allows following five cellular parameters: cell shape, MT array, nucleus position, nucleolus position, and chromatin condensation. We find that the states of these parameters are not randomly associated and identify 11 cellular states, referred to as landmarks, which occur much more frequently than closely related ones, indicating that they are convergence points during meiotic progression. As a first application of our system, we revisited a previously identified mutant in the meiotic A-type cyclin TARDY ASYNCHRONOUS MEIOSIS (TAM). Our imaging system enabled us to reveal both qualitatively and quantitatively altered landmarks in tam, foremost the formation of previously not recognized ectopic spindle- or phragmoplast-like structures that arise without attachment to chromosomes. en
dc.language.iso en en
dc.publisher Tools and Resources en
dc.rights © 2019, Prusicki et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. ja
dc.title Live cell imaging of meiosis in Arabidopsis thaliana en
dc.type.nii Journal Article en
dc.contributor.transcription コマキ, シンイチロウ ja
dc.contributor.alternative 小牧, 伸一郎 ja
dc.textversion publisher en
dc.identifier.jtitle eLife en
dc.identifier.volume 8 en
dc.relation.doi 10.7554/eLife.42834.001 en
dc.identifier.artnum e42834 en
dc.identifier.NAIST-ID 74654146 en

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