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The unfolded protein response alongside the diauxic shift of yeast cells and its involvement in mitochondria enlargement

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dc.contributor.author Duc, Minh Tran en
dc.contributor.author Ishiwata-Kimata, Yuki en
dc.contributor.author Thanh, Chi Mai en
dc.contributor.author Kubo, Minoru en
dc.contributor.author Kimata, Yukio en
dc.date.accessioned 2019-10-31T09:02:43Z en
dc.date.available 2019-10-31T09:02:43Z en
dc.date.issued 2019-09-04 en
dc.identifier.issn 2045-2322 en
dc.identifier.uri http://hdl.handle.net/10061/13407 en
dc.description.abstract Upon dysfunction of the endoplasmic reticulum (ER), eukaryotic cells evoke the unfolded protein response (UPR), which, in yeast Saccharomyces cerevisaie cells, is promoted by the ER-located transmembrane endoribonuclease Ire1. When activated, Ire1 splices and matures the HAC1 mRNA which encodes a transcription-factor protein that is responsible for the gene induction of the UPR. Here we propose that this signaling pathway is also used in cellular adaptation upon diauxic shift, in which cells shift from fermentative phase (fast growth) to mitochondrial respiration phase (slower growth). Splicing of the HAC1 mRNA was induced upon diauxic shift of cells cultured in glucose-based media or in cells transferred from glucose-based medium to non-fermentable glycerol-based medium. Activation of Ire1 in this situation was not due to ER accumulation of unfolded proteins, and was mediated by reactive oxygen species (ROS), which are byproducts of aerobic respiration. Here we also show that the UPR induced by diauxic shift causes enlargement of the mitochondria, and thus contributes to cellular growth under non-fermentative conditions, in addition to transcriptional induction of the canonical UPR target genes, which includes those encoding ER-located molecular chaperones and protein-folding enzymes. en
dc.language.iso en en
dc.publisher nature en
dc.subject Fungal biology en
dc.subject Stress signalling en
dc.title The unfolded protein response alongside the diauxic shift of yeast cells and its involvement in mitochondria enlargement en
dc.type.nii Journal Article en
dc.contributor.transcription クボ, ミノル ja
dc.contributor.alternative 久保, 稔 ja
dc.contributor.alternative 木俣, 行雄 ja
dc.textversion publisher en
dc.identifier.jtitle Scientific Reports en
dc.relation.doi 10.1038/s41598-019-49146-5 en
dc.identifier.artnum 12780 en
dc.identifier.NAIST-ID 85624005 en
dc.identifier.NAIST-ID 74650268 en
dc.identifier.NAIST-ID 73290140 en


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