Rational Design of Domain‐Swapping‐Based c‐Type Cytochrome Heterodimers by Using Chimeric Proteins

Zhang, Mohan; Nakanishi, Tsukasa; Yamanaka, Masaru; Nagao, Satoshi; Yanagisawa, Sachiko; Shomura, Yasuhito; Shibata, Naoki; Ogura, Takashi; Higuchi, Yoshiki; Hirota, Shun

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Rational Design of Domain‐Swapping‐Based c‐Type Cytochrome Heterodimers by Using Chimeric Proteins

Zhang, Mohan; Nakanishi, Tsukasa; Yamanaka, Masaru; Nagao, Satoshi; Yanagisawa, Sachiko; Shomura, Yasuhito; Shibata, Naoki; Ogura, Takashi; Higuchi, Yoshiki; Hirota, Shun

URI: http://hdl.handle.net/10061/13382

Date: 2017-09-05

Abstract:

Swap shop: A new methodology is applied in the construction of c‐type cytochrome (cyt) heterodimers through the domain swapping of two chimeric proteins, in which the N‐terminal regions of Pseudomonas aeruginosa cyt c551 and Hydrogenobacter thermophilus cyt c552 are exchanged with each other. The result is a heterodimer with different active sites.

Information:

Version: author

Journal title: RationalChemBioChem

Vol(Issue): 18 (17)

Page: 1712-1715

Language: en

Original DOI: https://doi.org/10.1002/cbic.201700219

Copyright: © 2017 Wiley‐VCH Verlag GmbH & Co. KGaA, WeinheimThis is the peer reviewed version of the following article: ChemBioChem/ Volume 18, Issue 17, which has been published in final form at 10.1002/cbic.201700219. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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