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Structural Basis for the Function of the β-Barrel Assembly-Enhancing Protease BepA

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dc.contributor.author Tanaka, Yoshiki en
dc.contributor.author Hayashi, Yugo en
dc.contributor.author Kamikubo, Hironari en
dc.contributor.author Tsukazaki, Tomoya en
dc.date.accessioned 2019-05-16T06:19:45Z en
dc.date.available 2019-05-16T06:19:45Z en
dc.date.issued 2019-02-01 en
dc.identifier.issn 0022-2836 en
dc.identifier.uri http://hdl.handle.net/10061/13292 en
dc.description 出版社許諾条件により、本文は2020年2月1日以降に公開 ja
dc.description.abstract The β-barrel assembly machinery (BAM) complex mediates the assembly of β-barrel membrane proteins in the outer membrane. BepA, formerly known as YfgC, interacts with the BAM complex and functions as a protease/chaperone for the enhancement of the assembly and/or degradation of β-barrel membrane proteins. To elucidate the molecular mechanism underlying the dual functions of BepA, its full-length three-dimensional structure is needed. Here, we report the crystal structure of full-length BepA at 2.6-Å resolution. BepA possesses an N-terminal protease domain and a C-terminal tetratricopeptide repeat domain, which interact with each other. Domain cross-linking by structure-guided introduction of disulfide bonds did not affect the activities of BepA in vivo, suggesting that the function of this protein does not involve domain rearrangement. The full-length BepA structure is compatible with the previously proposed docking model of BAM complex and tetratricopeptide repeat domain of BepA. ja
dc.language.iso en en
dc.publisher Elsevier en
dc.rights © 2018 Elsevier Ltd. All rights reserved. ja
dc.subject outer membrane; en
dc.subject protein biogenesis; en
dc.subject protease en
dc.subject chaperon en
dc.subject crystal structure en
dc.title Structural Basis for the Function of the β-Barrel Assembly-Enhancing Protease BepA ja
dc.type.nii Journal Article en
dc.contributor.transcription タナカ, ヨシキ ja
dc.contributor.transcription ハヤシ, ユウゴ ja
dc.contributor.transcription カミクボ, ヒロナリ ja
dc.contributor.transcription ツカザキ, トモヤ ja
dc.contributor.alternative 田中, 良樹 ja
dc.contributor.alternative 林, 有吾 ja
dc.contributor.alternative 上久保, 裕生 ja
dc.contributor.alternative 塚﨑, 智也 ja
dc.textversion author en
dc.identifier.jtitle Journal of Molecular Biology en
dc.identifier.volume 431 en
dc.identifier.issue 3 en
dc.identifier.spage 625 en
dc.identifier.epage 635 en
dc.relation.doi 10.1016/j.jmb.2018.11.024 en
dc.identifier.NAIST-ID 74650169 en
dc.identifier.NAIST-ID 82041351 en
dc.identifier.NAIST-ID 73293268 en
dc.identifier.NAIST-ID 73299737 en
dc.relation.pmid 30521812 en

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