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Time-lapse observation of stepwise regression of Erk activity in zebrafish presomitic mesoderm

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dc.contributor.author Sari, Dini Wahyu Kartika en
dc.contributor.author Akiyama, Ryutaro en
dc.contributor.author Honda, Naoki en
dc.contributor.author Ishijima, Hannosuke en
dc.contributor.author Bessho, Yasumasa en
dc.contributor.author Matsui, Takaaki en
dc.date.accessioned 2019-02-19T05:14:16Z en
dc.date.available 2019-02-19T05:14:16Z en
dc.date.issued 2018-03-12 en
dc.identifier.uri http://hdl.handle.net/10061/13119 en
dc.description.abstract During somite segmentation, clock genes oscillate within the posterior presomitic mesoderm (PSM). The temporal information ties up with the posteriorly moving FGF gradient, leading to the formation of a presumptive somite within the PSM. We previously investigated Erk activity downstream of FGF signaling by collecting stained zebrafish embryos, and discovered that the steep gradient of Erk activity was generated in the PSM, and the Erk activity border regularly shifted in a stepwise manner. However, since these interpretations come from static analyses, we needed to firmly confirm them by applying an analysis that has higher spatiotemporal resolutions. Here we developed a live imaging system for Erk activity in zebrafish embryos, using a Förster resonance energy transfer (FRET)-based Erk biosensor. With this system, we firmly showed that Erk activity exhibits stepwise regression within the PSM. Although our static analyses could not detect the stepwise pattern of Erk activity in clock-deficient embryos, our system revealed that, in clock-deficient embryos, the stepwise regression of Erk activity occurs at an irregular timing, eventually leading to formation of irregularly-sized somites. Therefore, our system overcame the limitation of static analyses and revealed that clock-dependent spatiotemporal regulation of Erk is required for proper somitogenesis in zebrafish. ja
dc.language.iso en en
dc.publisher Springer Nature en
dc.rights @The author(s) 2018 en
dc.title Time-lapse observation of stepwise regression of Erk activity in zebrafish presomitic mesoderm en
dc.type.nii Journal Article en
dc.contributor.transcription アキヤマ, リュウタロウ ja
dc.contributor.transcription ベッショ, ヤスマサ ja
dc.contributor.transcription マツイ, タカキ ja
dc.contributor.alternative 秋山, 隆太郎 ja
dc.contributor.alternative 別所, 康全 ja
dc.contributor.alternative 松井, 貴輝 ja
dc.textversion publisher en
dc.identifier.abstracturl https://www.nature.com/articles/s41598-018-22619-9#Abs1 en
dc.identifier.jtitle Scientific Reports en
dc.identifier.volume 8 en
dc.identifier.issue 1 en
dc.relation.doi 10.1038/s41598-018-22619-9 en
dc.identifier.artnum 4335 en
dc.identifier.NAIST-ID 82040809 en
dc.identifier.NAIST-ID 73290454 en
dc.identifier.NAIST-ID 73290470 en
dc.relation.pmid 29531317 en


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