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Nutrient Signaling via the TORC1-Greatwall-PP2AB55δ Pathway Responsible for the High Initial Rates of Alcoholic Fermentation in Sake Yeast Strains of Saccharomyces cerevisiae

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dc.contributor.author Watanabe, Daisuke en
dc.contributor.author Kajihara, Takuma en
dc.contributor.author Sugimoto, Yukiko en
dc.contributor.author Takagi, Kenichi en
dc.contributor.author Mizuno, Megumi en
dc.contributor.author Zhou, Yan en
dc.contributor.author Chen, Jiawen en
dc.contributor.author Takeda, Kojiro en
dc.contributor.author Tatebe, Hisashi en
dc.contributor.author Shiozaki, Kazuhiro en
dc.contributor.author Nakazawa, Nobushige en
dc.contributor.author Izawa, Shingo en
dc.contributor.author Akao, Takesh en
dc.contributor.author Shimoi, Hitoshi en
dc.contributor.author Maeda, Tatsuya en
dc.contributor.author Takagi, Hiroshi en
dc.date.accessioned 2018-10-23T02:47:56Z en
dc.date.available 2018-10-23T02:47:56Z en
dc.date.issued 2018-10-19 en
dc.identifier.issn 0099-2240 en
dc.identifier.uri http://hdl.handle.net/10061/12605 en
dc.description.abstract Saccharomyces cerevisiae sake yeast strain Kyokai no. 7 (K7) and its relatives carry a homozygous loss-of-function mutation in the RIM15 gene, which encodes a Greatwall-family protein kinase. Disruption of RIM15 in non-sake yeast strains leads to improved alcoholic fermentation, indicating that the defect in Rim15p is associated with the enhanced fermentation performance of sake yeast cells. In order to understand how Rim15p mediates fermentation control, we here focused on target-of-rapamycin protein kinase complex 1 (TORC1) and protein phosphatase 2A with the B55δ regulatory subunit (PP2AB55δ), complexes that are known to act upstream and downstream of Rim15p, respectively. Several lines of evidence, including our previous transcriptomic analysis data, suggested enhanced TORC1 signaling in sake yeast cells during sake fermentation. Fermentation tests of the TORC1-related mutants using a laboratory strain revealed that TORC1 signaling positively regulates the initial fermentation rate in a Rim15p-dependent manner. Deletion of the CDC55 gene encoding B55δ abolished the high fermentation performance of Rim15p-deficient laboratory yeast and sake yeast cells, indicating that PP2AB55δ mediates the fermentation control by TORC1 and Rim15p. The TORC1-Greatwall-PP2AB55δ pathway similarly affected the fermentation rate in the fission yeast Schizosaccharomyces pombe, strongly suggested that the evolutionarily conserved pathway governs alcoholic fermentation in yeasts. It is likely that elevated PP2AB55δ activity accounts for the high fermentation performance of sake yeast cells. Heterozygous loss-of-function mutations in CDC55 found in K7-related sake strains may indicate that the Rim15p-deficient phenotypes are disadvantageous to cell survival. ja
dc.language.iso en en
dc.publisher American Society for Microbiology en
dc.rights © 2018 American Society for Microbiology. ja
dc.rights 出版社許諾条件により、本文は2019年4月19日以降に公開 ja
dc.subject Alcoholic fermentation en
dc.subject TORC1 en
dc.subject Greatwall en
dc.subject Rim15p en
dc.subject PP2AB55δ ja
dc.subject Cdc55p en
dc.subject sake yeast en
dc.subject Saccharomyces cerevisiae en
dc.subject Schizosaccharomyces pombe en
dc.title Nutrient Signaling via the TORC1-Greatwall-PP2AB55δ Pathway Responsible for the High Initial Rates of Alcoholic Fermentation in Sake Yeast Strains of Saccharomyces cerevisiae ja
dc.type.nii Journal Article en
dc.contributor.transcription ワタナベ, ダイスケ ja
dc.contributor.transcription タテベ, ヒサシ ja
dc.contributor.transcription シオザキ, カズヒロ ja
dc.contributor.transcription タカギ, ヒロシ ja
dc.contributor.alternative 渡辺, 大輔 ja
dc.contributor.alternative 建部, 恒 ja
dc.contributor.alternative 塩崎, 一裕 ja
dc.contributor.alternative 髙木, 博史 ja
dc.textversion publisher en
dc.identifier.ncid AA00543249 en
dc.identifier.jtitle Applied and Environmental Microbiology en
dc.relation.doi 10.1128/AEM.02083-18 en
dc.identifier.NAIST-ID 74650151 en
dc.identifier.NAIST-ID 73296634 en
dc.identifier.NAIST-ID 73296618 en
dc.identifier.NAIST-ID 73290561 en
dc.relation.pmid 30341081 en

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