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The Arabidopsis cyclin-dependent kinase-activating kinase CDKF;1 is a major regulator of cell proliferation and cell expansion but is dispensable for CDKA activation

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dc.contributor.author Takatsuka, Hirotomo en
dc.contributor.author Ohno, Ryoko en
dc.contributor.author Umeda, Masaaki en
dc.date.accessioned 2018-10-05T05:29:27Z en
dc.date.available 2018-10-05T05:29:27Z en
dc.date.issued 2009-07-24 en
dc.identifier.issn 0960-7412 en
dc.identifier.uri http://hdl.handle.net/10061/12591 en
dc.description.abstract Summary Cyclin-dependent kinases (CDKs) play an essential role in cell cycle regulation during the embryonic and post-embryonic development of various organisms. Full activation of CDKs requires not only binding to cyclins but also phosphorylation of the T-loop domain. This phosphorylation is catalysed by CDK-activating kinases (CAKs). Plants have two distinct types of CAKs, namely CDKD and CDKF; in Arabidopsis, CDKF;1 exhibits the highest CDK kinase activity in vitro. We have previously shown that CDKF;1 also functions in the activation of CDKD;2 and CDKD;3 by T-loop phosphorylation. Here, we isolated the knockout mutants of CDKF;1 and showed that they had severe defects in cell division, cell elongation and endoreduplication. No defect was observed during embryogenesis, suggesting that CDKF;1 function is primarily required for post-embryonic development. In the cdkf;1 mutants, T-loop phosphorylation of CDKA;1, an orthologue of yeast Cdc2/Cdc28p, was comparable to that in wild-type plants, and its kinase activity did not decrease. In contrast, the protein level and kinase activity of CDKD;2 were significantly reduced in the mutants. Substitution of threonine-168 with a non-phosphorylatable alanine residue made CDKD;2 unstable in Arabidopsis tissues. These results indicate that CDKF;1 is dispensable for CDKA;1 activation but is essential for maintaining a steady-state level of CDKD;2, thereby suggesting the quantitative regulation of a vertebrate-type CAK in a plant-specific manner. en
dc.language.iso en en
dc.publisher Wiley/Blackwell (10.1111) en
dc.rights © 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd ja
dc.rights This is the peer reviewed version of the following article: [The Plant Journal, 59(3):475-487], which has been published in final form at [https://doi.org/10.1111/j.1365-313X.2009.03884.x]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. en
dc.subject cyclin-dependent kinase en
dc.subject CDK-activating kinase en
dc.subject cell cycle en
dc.subject transcription en
dc.subject protein phosphorylation en
dc.subject protein stability en
dc.title The Arabidopsis cyclin-dependent kinase-activating kinase CDKF;1 is a major regulator of cell proliferation and cell expansion but is dispensable for CDKA activation en
dc.type.nii Journal Article en
dc.contributor.transcription ウメダ, マサアキ ja
dc.contributor.alternative 梅田, 正明 ja
dc.textversion author en
dc.identifier.ncid AA10846815 en
dc.identifier.jtitle The Plant Journal en
dc.identifier.volume 59 en
dc.identifier.issue 3 en
dc.identifier.spage 475 en
dc.identifier.epage 487 en
dc.relation.doi 10.1111/j.1365-313X.2009.03884.x en
dc.identifier.NAIST-ID 73290587 en
dc.relation.pmid 19368694 en

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