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Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

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dc.contributor.author Adachi, Sumiko en
dc.contributor.author Nobusawa, Takashi en
dc.contributor.author Umeda, Masaaki en
dc.date.accessioned 2018-10-04T05:34:25Z en
dc.date.available 2018-10-04T05:34:25Z en
dc.date.issued 2009-05-15 en
dc.identifier.issn 0012-1606 en
dc.identifier.uri http://hdl.handle.net/10061/12581 en
dc.description.abstract A-type cyclin-dependent kinase (CDKA) is an ortholog of yeast Cdc2/Cdc28p, and is assumed to have an essential function in plant growth and organogenesis. Previous studies revealed that its kinase activity is controlled by post-translational modifications, such as binding to cyclins and phosphorylations, but its transcriptional regulation is poorly understood. Here, we generated a promoter dissection series of Arabidopsis (Arabidopsis thaliana) CDKA;1, and used β-glucuronidase (GUS) gene-fused reporter constructs for expression analyses in planta. The results revealed two types of transcriptional control in shoots: general quantitative regulation and cell type-specific regulation. We identified a promoter region that promotes CDKA;1 expression in the leaf epidermis, but not in the L1 layer of the shoot apical meristem. This region also directed abaxial side-biased expression, which may be linked to the adaxial/abaxial side specification. Another reporter construct showed that CDKA;1 expression in the inner layers of leaves is controlled by a distinct regulatory region in the promoter. These results suggest that the transcriptional regulation of CDKA;1 may play a key role in proper development of leaves by coordinating cell division and differentiation of different cell types. ja
dc.language.iso en en
dc.publisher Elsevier en
dc.rights © 2009 Elsevier Inc. ja
dc.subject Cyclin-dependent kinase en
dc.subject Cell division en
dc.subject Epidermis en
dc.subject Gene expression en
dc.title Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana en
dc.type.nii Journal Article en
dc.contributor.transcription ウメダ, マサアキ ja
dc.contributor.alternative 梅田, 正明 ja
dc.textversion author en
dc.identifier.ncid AA00627848 en
dc.identifier.jtitle Developmental Biology en
dc.identifier.volume 329 en
dc.identifier.issue 2 en
dc.identifier.spage 306 en
dc.identifier.epage 314 en
dc.relation.doi 10.1016/j.ydbio.2009.03.002 en
dc.identifier.NAIST-ID 73290587 en
dc.relation.pmid 19285489 en


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