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Identifying the target genes of SUPPRESSOR OF GAMMA RESPONSE 1, a master transcription factor controlling DNA damage response in Arabidopsis

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dc.contributor.author Ogita, Nobuo en
dc.contributor.author Okushima, Yoko en
dc.contributor.author Tokizawa, Mutsutomo en
dc.contributor.author Yamamoto, Yoshiharu Y. en
dc.contributor.author Tanaka, Maho en
dc.contributor.author Seki, Motoaki en
dc.contributor.author Makita, Yuko en
dc.contributor.author Matsui, Minami en
dc.contributor.author Okamoto Yoshiyama, Kaoru en
dc.contributor.author Sakamoto, Tomoaki en
dc.contributor.author Kurata, Tetsuya en
dc.contributor.author Hiruma, Kei en
dc.contributor.author Saijo, Yusuke en
dc.contributor.author Takahashi, Naoki en
dc.contributor.author Umeda, Masaaki en
dc.date.accessioned 2018-03-01T07:28:43Z en
dc.date.available 2018-03-01T07:28:43Z en
dc.date.issued 2018-02-11 en
dc.identifier.issn 1365-313X en
dc.identifier.uri http://hdl.handle.net/10061/12209 en
dc.description.abstract In mammalian cells, the transcription factor p53 plays a crucial role in transmitting DNA damage signals to maintain genome integrity. However, in plants, orthologous genes for p53 and checkpoint proteins are absent. Instead, the plant-specific transcription factor SUPPRESSOR OF GAMMA RADIATION 1 (SOG1) controls most of the genes induced by gamma irradiation and promotes DNA repair, cell cycle arrest, and stem cell death. Thus far, the genes directly controlled by SOG1 remain largely unknown, limiting the understanding of DNA damage signaling in plants. Here, we conducted a microarray analysis and chromatin immunoprecipitation (ChIP)-sequencing, and identified 146 Arabidopsis genes as direct targets of SOG1. By using the ChIP-sequencing data, we extracted the palindromic motif [CTT(N)7AAG] as a consensus SOG1-binding sequence, which mediates target gene induction in response to DNA damage. Furthermore, DNA damage-triggered phosphorylation of SOG1 is required for efficient binding to SOG1-binding sequence. Comparison between SOG1 and p53 target genes showed that both transcription factors control genes responsible for cell cycle regulation, such as CDK inhibitors, and DNA repair proteins, whereas SOG1 preferentially targets genes involved in homologous recombination. We also found that defense-related genes were enriched in the SOG1 target genes. Consistent with this, SOG1 is required for resistance against the hemi-biotrophic fungus Colletotrichum higginsianum, suggesting that SOG1 has a unique function in controlling immune response. This article is protected by copyright. All rights reserved. en
dc.language.iso en en
dc.publisher Wiley Online Library en
dc.rights Copyright © 1999 - 2018 John Wiley & Sons, Inc ja
dc.rights 出版社許諾条件により、本文は2019年2月11日以降に公開。 ja
dc.subject Arabidopsis thaliana en
dc.subject ChIP-Seq en
dc.subject cis-element en
dc.subject DNA damage response en
dc.subject immune response en
dc.subject NAC transcription factor en
dc.subject p53 en
dc.subject SOG1 en
dc.title Identifying the target genes of SUPPRESSOR OF GAMMA RESPONSE 1, a master transcription factor controlling DNA damage response in Arabidopsis en
dc.type.nii Journal Article en
dc.contributor.transcription オギタ, ノブオ ja
dc.contributor.transcription オクシマ, ヨウコ ja
dc.contributor.transcription サカモト, トモアキ ja
dc.contributor.transcription クラタ, テツヤ ja
dc.contributor.transcription ヒルマ, ケイ ja
dc.contributor.transcription サイジョウ, ユウスケ ja
dc.contributor.transcription タカハシ, ナオキ ja
dc.contributor.transcription ウメダ, マサアキ ja
dc.contributor.alternative 荻田, 伸夫 ja
dc.contributor.alternative 奥島, 葉子 ja
dc.contributor.alternative 坂本, 智昭 ja
dc.contributor.alternative 倉田, 哲也 ja
dc.contributor.alternative 晝間, 敬 ja
dc.contributor.alternative 西條, 雄介 ja
dc.contributor.alternative 高橋, 直紀 ja
dc.contributor.alternative 梅田, 正明 ja
dc.textversion author en
dc.identifier.abstracturl http://onlinelibrary.wiley.com/doi/10.1111/tpj.13866/abstract en
dc.identifier.jtitle The Plant Journal en
dc.relation.doi 10.1111/tpj.13866 en
dc.identifier.NAIST-ID 83515221 en
dc.identifier.NAIST-ID 73290553 en
dc.identifier.NAIST-ID 73294555 en
dc.identifier.NAIST-ID 73294506 en
dc.identifier.NAIST-ID 74650425 en
dc.identifier.NAIST-ID 73299711 en
dc.identifier.NAIST-ID 73294654 en
dc.identifier.NAIST-ID 73290587 en
dc.relation.pmid 29430765 en

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