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Characterization of the Cytochrome c Membrane-Binding Site Using Cardiolipin-Containing Bicelles with NMR

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dc.contributor.author Kobayashi, Hisashi en
dc.contributor.author Nagao, Satoshi en
dc.contributor.author Hirota, Shun en
dc.date.accessioned 2016-10-14T04:47:12Z en
dc.date.available 2016-10-14T04:47:12Z en
dc.date.issued 2016-10-10 en
dc.identifier.issn 1521-3757 en
dc.identifier.uri http://hdl.handle.net/10061/11025 en
dc.description.abstract Cytochrome (cyt) c transports electrons from Complex III to Complex IV in mitochondria. Cyt c is ordinarily anchored to the mitochondrial membrane through interaction with cardiolipin (CL), however its release into the cytosol initiates apoptosis. The cyt c interaction site with CL-containing bicelles was characterized by NMR spectroscopy. Chemical shift perturbations in cyt c signals upon interaction with bicelles revealed that a relatively wide region, which includes the A-site, the CXXCH motif, and the N- and C-terminal helices, and contains multiple Lys residues, interacts cooperatively with CL. The specific cyt c–CL interaction increased with increasing CL molecules in the bicelles. The location of the cyt c interaction site for CL was similar to those for Complex III and Complex IV, thus indicating that cyt c recognizes lipids and partner proteins in a similar way. In addition to elucidating the cyt c membrane-binding site, these results provide insight into the dynamic aspect of cyt c interactions in mitochondria. ja
dc.language.iso en en
dc.publisher Wiley-VCH Verlag GmbH & Co en
dc.rights © 1999 - 2016 John Wiley & Sons, Inc. ja
dc.rights This is the accepted version of the following article:Characterization of the Cytochrome c Membrane-Binding Site Using Cardiolipin-Containing Bicelles with NMR, Hisashi Kobayashi,Satoshi Nagao, Shun Hirota, Angewandte Chemie, Oct 10, 2016, which has been published in final form at http://dx.doi.org/10.1002/anie.201607419. ja
dc.rights 出版社許諾条件により本文は2017年10月10日以降公開。 ja
dc.subject Bicellen en
dc.subject Cytochrom c ja
dc.subject Membranproteine en
dc.subject NMR-Spektroskopie en
dc.subject Protein-Lipid-Wechselwirkungen en
dc.title Characterization of the Cytochrome c Membrane-Binding Site Using Cardiolipin-Containing Bicelles with NMR ja
dc.type.nii Journal Article en
dc.contributor.transcription コバヤシ, ヒサシ ja
dc.contributor.transcription ナガオ, サトシ ja
dc.contributor.transcription ヒロタ, シュン ja
dc.contributor.alternative 小林, 紀 ja
dc.contributor.alternative 長尾, 聡 ja
dc.contributor.alternative 廣田, 俊 ja
dc.textversion author en
dc.identifier.ncid AA11614145 en
dc.identifier.jtitle Angewandte Chemie en
dc.relation.doi 10.1002/anie.201607419 en
dc.identifier.NAIST-ID 73293425 en
dc.identifier.NAIST-ID 83516492 en
dc.identifier.NAIST-ID 73293409 en
dc.relation.pmid 27723218 en


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