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Integrating reductive and synthetic approaches in biology using man-made cell-like compartments

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dc.contributor.author Aoki, Wataru en
dc.contributor.author Saito, Masato en
dc.contributor.author Manabe, Ri-ichiroh en
dc.contributor.author Mori, Hirotada en
dc.contributor.author Yamaguchi, Yoshinori en
dc.contributor.author Tamiya, Eiichi en
dc.date.accessioned 2016-08-19T07:43:52Z en
dc.date.available 2016-08-19T07:43:52Z en
dc.date.issued 2014-04-17 en
dc.identifier.issn 2045-2322 en
dc.identifier.uri http://hdl.handle.net/10061/10980 en
dc.description.abstract We propose ‘integrated synthetic genetics’ as a novel methodology that integrates reductive and synthetic approaches used in life science research. Integrated synthetic genetics enables determinations of sets of genes required for the functioning of any biological subsystem. This method utilizes artificial cell-like compartments, including a randomly introduced whole gene library, strictly defined components for in vitro transcription and translation and a reporter that fluoresces ‘only when a particular function of a target biological subsystem is active.’ The set of genes necessary for the target biological subsystem can be identified by isolating fluorescent artificial cells and multiplex next-generation sequencing of genes included in these cells. The importance of this methodology is that screening for the set of genes involved in a subsystem and reconstructing the entire subsystem can be done simultaneously. This methodology can be applied to any biological subsystem of any species and may remarkably accelerate life science research. ja
dc.language.iso en en
dc.publisher Nature Publishing Group en
dc.rights c 2016 Macmillan Publishers Limited, part of Springer Nature. All rights reserved. en
dc.title Integrating reductive and synthetic approaches in biology using man-made cell-like compartments en
dc.type.nii Journal Article en
dc.contributor.transcription モリ, ヒロタダ ja
dc.contributor.alternative 森, 浩禎 ja
dc.identifier.jtitle Scientific Reports en
dc.identifier.volume 4 en
dc.identifier.spage 4722 en
dc.relation.doi 10.1038/srep04722 en
dc.identifier.NAIST-ID 73290108 en
dc.relation.isIdenticalTo http://www.nature.com/articles/srep04722 en

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