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Title: Astrocyte-Specific Genes Are Generally Demethylated in Neural Precursor Cells Prior to Astrocytic Differentiation
Authors: Izuho Hatada
Masakazu Namihira
Sumiyo Morita
Mika Kimura
Takuro Horii
Kinichi Nakashima
Issue Date: 11-Sep-2008
Publisher: Public Library of Science
Journal Title: PLOS ONE
Volume: 3
Issue: 9
Article Number: e3189
Abstract: Epigenetic changes are thought to lead to alterations in the property of cells, such as differentiation potential. Neural precursor cells (NPCs) differentiate only into neurons in the midgestational brain, yet they become able to generate astrocytes in the late stage of development. This differentiation-potential switch could be explained by epigenetic changes, since the promoters of astrocyte-specific marker genes, glial fibrillary acidic protein (Gfap) and S100b, have been shown to become demethylated in late-stage NPCs prior to the onset of astrocyte differentiation; however, whether demethylation occurs generally in other astrocyctic genes remains unknown. Here we analyzed DNA methylation changes in mouse NPCs between the mid-(E11.5) and late (E14.5) stage of development by a genome-wide DNA methylation profiling method using microarrays and found that many astrocytic genes are demethylated in late-stage NPCs, enabling the cell to become competent to express these genes. Although these genes are already demethylated in late-stage NPCs, they are not expressed until cells differentiate into astrocytes. Thus, late-stage NPCs have epigenetic potential which can be realized in their expression after astrocyte differentiation.
ISSN: 1932-6203
Rights: Copyright (C) 2008 Public Library of Science.
Text Version: Publisher
Publisher DOI: 10.1371/journal.pone.0003189
Appears in Collections:バイオサイエンス研究科 / Graduate School of Biological Sciences

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