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Title: The Escherichia coli K-12 ORFeome: a resource for comparative molecular microbiology
Authors: Rajagopala, Seesandra V.
Yamamoto, Natsuko
Zweifel, Adrienne E.
Nakamichi, Tomoko
Huang, Hsi-Kuang
Mendez-Rios, Jorge David
Franca-Koh, Jonathan
Boorgula, Meher Preethi
Fujita, Kazutoshi
Suzuki, Ken-ichirou
Hu, James C.
Wanner, Barry L.
Mori, Hirotada
Uetz, Peter
Issue Date: 11-Aug-2010
Publisher: biomed central
Journal Title: BMC GENOMICS
Volume: 11
Article Number: 470
Abstract: Background:
Systems biology and functional genomics require genome-wide datasets and resources. Complete sets of cloned open reading frames (ORFs) have been made for about a dozen bacterial species and allow researchers to express and study complete proteomes in a high-throughput fashion.
We have constructed an open reading frame (ORFeome) collection of 3974 or 94% of the known Escherichia coli K-12 ORFs in Gateway® entry vector pENTR/Zeo. The collection has been used for protein expression and protein interaction studies. For example, we have compared interactions among YgjD, YjeE and YeaZ proteins in E. coli, Streptococcus pneumoniae, and Staphylococcus aureus. We also compare this ORFeome with other Gateway-compatible bacterial ORFeomes and show its utility for comparative functional genomics.
The E. coli ORFeome provides a useful resource for functional genomics and other areas of protein research in a highly flexible format. Our comparison with other ORFeomes makes comparative analyses straighforward and facilitates direct comparisons of many proteins across many genomes.
ISSN: 1471-2164
Rights: Copyright (C) 2010 biomed central .
Publisher DOI: 10.1186/1471-2164-11-470
Appears in Collections:バイオサイエンス研究科 / Graduate School of Biological Sciences

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