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Title: HMG Domain Containing SSRP1 Is Required for DNA Demethylation and Genomic Imprinting in Arabidopsis
Authors: Ikeda, Yoko
Kinoshita, Yuki
Susaki, Daichi
Ikeda, Yuriko
Iwano, Megumi
Takayama, Seiji
Higashiyama, Tetsuya
Kakutani, Tetsuji
Kinoshita, Tetsu
Keywords: DNA demethylation
genomic imprinting
gene activation
Arabidopsis endosperm
Issue Date: Sep-2011
Publisher: Elsevier (Cell Press)
Volume: 21
Issue: 3
Start page: 589
End page: 596
Abstract: In Arabidopsis, DEMETER (DME) DNA demethylase contributes to reprogramming of the epigenetic state of the genome in the central cell. However, other aspects of the active DNA demethylation processes remain elusive. Here we show that Arabidopsis SSRP1, known as an HMG domain-containing component of FACT histone chaperone, is required for DNA demethylation and for activation and repression of many parentally imprinted genes in the central cell. Although loss of DNA methylation releases silencing of the imprinted FWA-GFP, double ssrp1-3;met1-3 mutants surprisingly showed limited activation of maternal FWA-GFP in the central cell, and only became fully active after several nuclear divisions in the endosperm. This behavior was in contrast to the dme-1;met1 double mutant in which hypomethylation of FWA-GFP by met1 suppressed the DNA demethylation defect of dme-1. We propose that active DNA demethylation by DME requires SSRP1 function through a distinctly different process from direct DNA methylation control.
ISSN: 1534-5807
Rights: Copyright (C) 2011 Elsevier (Cell Press) .
Text Version: author
Publisher DOI: 10.1016/j.devcel.2011.08.013
Appears in Collections:バイオサイエンス研究科 / Graduate School of Biological Sciences

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