|
naistar (NAIST Academic Repository) >
学術リポジトリ naistar / NAIST Academic Repository naistar >
学術雑誌論文 / Journal Article >
バイオサイエンス研究科 / Graduate School of Biological Sciences >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10061/6333
|
| Title: | Canopy1, a positive feedback regulator of FGF signaling, controls progenitor cell clustering during Kupffer's vesicle organogenesis |
| Authors: | Matsui, Takaaki
Thitamadee, Siripong
Murata, Tomoko
Kakinuma, Hisaya
Nabetani, Takuji
Hirabayashi, Yoshio
Hirate, Yoshikazu
Okamoto, Hitoshi
Bessho, Yasumasa |
| Keywords: | Cell cluster formation
Left-right patterning
Canopy
Positive feedback loop |
| Issue Date: | 14-Jun-2011 |
| Publisher: | National Academy of Science |
| Journal Title: | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
| Volume: | 108 |
| Issue: | 24 |
| Start page: | 9881 |
| End page: | 9886 |
| Abstract: | The assembly of progenitor cells is a crucial step for organ formation during vertebrate development. Kupffer’s vesicle (KV) is a key organ required for the left-right asymmetric body plan in zebrafish, and is generated from a cluster of approximately 20 dorsal forerunner cells (DFCs). Although several genes are known to be involved in KV formation, how DFC clustering is regulated and how cluster formation then contributes to KV formation remain unclear. Here we show that positive feedback regulation of FGF signaling by Canopy1 (Cnpy1) controls DFC clustering without affecting DFC specification and DFC number. Cnpy1 positively regulates FGF signals within DFCs, which in turn promotes Cadherin1-mediated cell adhesion between adjacent DFCs to sustain cell cluster formation. When this FGF positive feedback loop is disrupted, the DFC cluster fails to form, eventually leading to KV malformation and defects in the establishment of laterality. Our results therefore uncover both a previously unidentified role of FGF signaling during vertebrate organogenesis and a regulatory mechanism underlying cell cluster formation, which is an indispensable step for formation of a functional KV and establishment of the left-right asymmetric body plan. |
| URI: | http://hdl.handle.net/10061/6333 |
| ISSN: | 0027-8424 |
| Rights: | Copyright (c) 2011 by the National Academy of Sciences |
| Text Version: | author |
| Publisher DOI: | 10.1073/pnas.1017248108 |
| Appears in Collections: | バイオサイエンス研究科 / Graduate School of Biological Sciences
|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
|
