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Structural Basis for the Function of the β-Barrel Assembly-Enhancing Protease BepA

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dc.contributor.author Tanaka, Yoshiki
dc.contributor.author Hayashi, Yugo
dc.contributor.author Kamikubo, Hironari
dc.contributor.author Tsukazaki, Tomoya
dc.date.accessioned 2019-05-16T06:19:45Z
dc.date.available 2019-05-16T06:19:45Z
dc.date.issued 2019-02-01
dc.identifier.issn 0022-2836
dc.identifier.uri http://hdl.handle.net/10061/13292
dc.description 出版社許諾条件により、本文は2020年2月1日以降に公開 ja_JP
dc.description.abstract The β-barrel assembly machinery (BAM) complex mediates the assembly of β-barrel membrane proteins in the outer membrane. BepA, formerly known as YfgC, interacts with the BAM complex and functions as a protease/chaperone for the enhancement of the assembly and/or degradation of β-barrel membrane proteins. To elucidate the molecular mechanism underlying the dual functions of BepA, its full-length three-dimensional structure is needed. Here, we report the crystal structure of full-length BepA at 2.6-Å resolution. BepA possesses an N-terminal protease domain and a C-terminal tetratricopeptide repeat domain, which interact with each other. Domain cross-linking by structure-guided introduction of disulfide bonds did not affect the activities of BepA in vivo, suggesting that the function of this protein does not involve domain rearrangement. The full-length BepA structure is compatible with the previously proposed docking model of BAM complex and tetratricopeptide repeat domain of BepA. ja_JP
dc.language.iso en ja_JP
dc.publisher Elsevier ja_JP
dc.rights © 2018 Elsevier Ltd. All rights reserved. ja_JP
dc.subject outer membrane; ja_JP
dc.subject protein biogenesis; ja_JP
dc.subject protease ja_JP
dc.subject chaperon ja_JP
dc.subject crystal structure ja_JP
dc.title Structural Basis for the Function of the β-Barrel Assembly-Enhancing Protease BepA ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription タナカ, ヨシキ
dc.contributor.transcription ハヤシ, ユウゴ
dc.contributor.transcription カミクボ, ヒロナリ
dc.contributor.transcription ツカザキ, トモヤ
dc.contributor.alternative 田中, 良樹
dc.contributor.alternative 林, 有吾
dc.contributor.alternative 上久保, 裕生
dc.contributor.alternative 塚﨑, 智也
dc.identifier.fulltexturl https://www.sciencedirect.com/science/article/pii/S0022283618310088 ja_JP
dc.textversion author ja_JP
dc.identifier.jtitle Journal of Molecular Biology ja_JP
dc.identifier.volume 431 ja_JP
dc.identifier.issue 3 ja_JP
dc.identifier.spage 625 ja_JP
dc.identifier.epage 635 ja_JP
dc.relation.doi info:doi/10.1016/j.jmb.2018.11.024 ja_JP
dc.identifier.NAIST-ID 74650169 ja_JP
dc.identifier.NAIST-ID 82041351 ja_JP
dc.identifier.NAIST-ID 73293268 ja_JP
dc.identifier.NAIST-ID 73299737 ja_JP
dc.relation.pmid 30521812 ja_JP

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