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2.8-Å crystal structure of Escherichia coli YidC revealing all core regions, including flexible C2 loop

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dc.contributor.author Tanaka, Yoshiki
dc.contributor.author Tsukazaki, Tomoya
dc.date.accessioned 2019-05-13T06:12:09Z
dc.date.available 2019-05-13T06:12:09Z
dc.date.issued 2018-10-20
dc.identifier.issn 0006-291X
dc.identifier.uri http://hdl.handle.net/10061/13291
dc.description 出版社許諾条件により、本文は2019年10月20日以降に公開
dc.description.abstract YidC/Alb3/Oxa1 family proteins are involved in the insertion and assembly of membrane proteins. The core five transmembrane regions of YidC, which are conserved in the protein family, form a positively charged cavity open to the cytoplasmic side. The cavity plays an important role in membrane protein insertion. In all reported structural studies of YidC, the second cytoplasmic loop (C2 loop) was disordered, limiting the understanding of its role. Here, we determined the crystal structure of YidC including the C2 loop at 2.8 Å resolution with R/Rfree = 21.8/27.5. This structure and subsequent molecular dynamics simulation indicated that the intrinsic flexible C2 loop covered the positively charged cavity. This crystal structure provides the coordinates of the complete core region including the C2 loop, which is valuable for further analyses of YidC. ja_JP
dc.language.iso en ja_JP
dc.publisher Elsevier ja_JP
dc.rights © 2018 Elsevier Inc. All rights reserved. ja_JP
dc.subject Crystal structure ja_JP
dc.subject YidC ja_JP
dc.subject Insertase ja_JP
dc.subject Chaperone ja_JP
dc.subject Membrane protein ja_JP
dc.title 2.8-Å crystal structure of Escherichia coli YidC revealing all core regions, including flexible C2 loop ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription タナカ, ヨシキ
dc.contributor.transcription ツカザキ, トモヤ
dc.contributor.alternative 田中, 良樹
dc.contributor.alternative 塚崎, 智也
dc.identifier.fulltexturl http://www.sciencedirect.com/science/article/pii/S0006291X18319612 ja_JP
dc.textversion author ja_JP
dc.identifier.jtitle Biochemical and Biophysical Research Communications ja_JP
dc.identifier.volume 505 ja_JP
dc.identifier.issue 1 ja_JP
dc.identifier.spage 141 ja_JP
dc.identifier.epage 145 ja_JP
dc.relation.doi info:doi/10.1016/j.bbrc.2018.09.043 ja_JP
dc.identifier.NAIST-ID 74650169 ja_JP
dc.identifier.NAIST-ID 73299737 ja_JP
dc.relation.pmid 30241934 ja_JP

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