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ERK Activity Dynamics during Zebrafish Embryonic Development

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dc.contributor.author Wong, Kah-Loon
dc.contributor.author Akiyama, Ryutaro
dc.contributor.author Bessho, Yasumasa
dc.contributor.author Matsui, Takaaki
dc.date.accessioned 2019-02-19T05:15:13Z
dc.date.available 2019-02-19T05:15:13Z
dc.date.issued 2018-12-28
dc.identifier.uri http://hdl.handle.net/10061/13121
dc.description.abstract During vertebrate development, extracellular signal-regulated kinase (ERK) is activated by growth factors such as fibroblast growth factor (FGF), and it regulates the formation of tissues/organs including eyes, brains, somites, limbs, and inner ears. However, an experimental system to monitor ERK activity dynamics in the entire body of the vertebrate embryo is lacking. We recently studied ERK activity dynamics in the pre-somitic mesoderm of living zebrafish embryos injected with mRNAs encoding a Förster resonance energy transfer (FRET)-based ERK biosensor. In this study, transgenic zebrafish stably and ubiquitously expressing the ERK biosensor were generated to monitor ERK activity dynamics throughout embryonic development. The system allowed the identification of ERK activation domains in embryos from the late blastula to the late segmentation stage, consistent with immunostaining patterns obtained using anti-phosphorylated ERK antibody. A spatiotemporal map of ERK activity in the entire body during zebrafish embryogenesis was generated, and previously unidentified activation dynamics and ERK domains were identified. The proposed system is the first reported method to monitor ERK activity dynamics during vertebrate embryogenesis, providing insight into the role of ERK activity in normal and abnormal development in living vertebrate embryos. ja_JP
dc.language.iso en ja_JP
dc.publisher MDPI ja_JP
dc.rights @ 2018 by the authors ja_JP
dc.subject mitogen-activated protein kinase (MAPK) ja_JP
dc.subject biosensor; signal activity ja_JP
dc.subject vertebrate development ja_JP
dc.title ERK Activity Dynamics during Zebrafish Embryonic Development ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription アキヤマ, リュウタロウ
dc.contributor.transcription ベッショ, ヤスマサ
dc.contributor.transcription マツイ, タカアキ
dc.contributor.alternative 秋山, 隆太郎
dc.contributor.alternative 別所, 康全
dc.contributor.alternative 松井, 貴輝
dc.identifier.fulltexturl https://doi.org/10.3390/ijms20010109 ja_JP
dc.textversion publisher ja_JP
dc.identifier.jtitle International Journal of Molecular Sciences ja_JP
dc.identifier.volume 20 ja_JP
dc.identifier.issue 1 ja_JP
dc.relation.doi info:doi/10.3390/ijms20010109 ja_JP
dc.identifier.artnum 109 ja_JP
dc.identifier.NAIST-ID 82040809 ja_JP
dc.identifier.NAIST-ID 73290454 ja_JP
dc.identifier.NAIST-ID 73290470 ja_JP
dc.relation.pmid 30597912 ja_JP

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