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Nutrient Signaling via the TORC1-Greatwall-PP2AB55δ Pathway Responsible for the High Initial Rates of Alcoholic Fermentation in Sake Yeast Strains of Saccharomyces cerevisiae

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dc.contributor.author Watanabe, Daisuke
dc.contributor.author Kajihara, Takuma
dc.contributor.author Sugimoto, Yukiko
dc.contributor.author Takagi, Kenichi
dc.contributor.author Mizuno, Megumi
dc.contributor.author Zhou, Yan
dc.contributor.author Chen, Jiawen
dc.contributor.author Takeda, Kojiro
dc.contributor.author Tatebe, Hisashi
dc.contributor.author Shiozaki, Kazuhiro
dc.contributor.author Nakazawa, Nobushige
dc.contributor.author Izawa, Shingo
dc.contributor.author Akao, Takesh
dc.contributor.author Shimoi, Hitoshi
dc.contributor.author Maeda, Tatsuya
dc.contributor.author Takagi, Hiroshi
dc.date.accessioned 2018-10-23T02:47:56Z
dc.date.available 2018-10-23T02:47:56Z
dc.date.issued 2018-10-19
dc.identifier.issn 0099-2240
dc.identifier.uri http://hdl.handle.net/10061/12605
dc.description.abstract Saccharomyces cerevisiae sake yeast strain Kyokai no. 7 (K7) and its relatives carry a homozygous loss-of-function mutation in the RIM15 gene, which encodes a Greatwall-family protein kinase. Disruption of RIM15 in non-sake yeast strains leads to improved alcoholic fermentation, indicating that the defect in Rim15p is associated with the enhanced fermentation performance of sake yeast cells. In order to understand how Rim15p mediates fermentation control, we here focused on target-of-rapamycin protein kinase complex 1 (TORC1) and protein phosphatase 2A with the B55δ regulatory subunit (PP2AB55δ), complexes that are known to act upstream and downstream of Rim15p, respectively. Several lines of evidence, including our previous transcriptomic analysis data, suggested enhanced TORC1 signaling in sake yeast cells during sake fermentation. Fermentation tests of the TORC1-related mutants using a laboratory strain revealed that TORC1 signaling positively regulates the initial fermentation rate in a Rim15p-dependent manner. Deletion of the CDC55 gene encoding B55δ abolished the high fermentation performance of Rim15p-deficient laboratory yeast and sake yeast cells, indicating that PP2AB55δ mediates the fermentation control by TORC1 and Rim15p. The TORC1-Greatwall-PP2AB55δ pathway similarly affected the fermentation rate in the fission yeast Schizosaccharomyces pombe, strongly suggested that the evolutionarily conserved pathway governs alcoholic fermentation in yeasts. It is likely that elevated PP2AB55δ activity accounts for the high fermentation performance of sake yeast cells. Heterozygous loss-of-function mutations in CDC55 found in K7-related sake strains may indicate that the Rim15p-deficient phenotypes are disadvantageous to cell survival. ja_JP
dc.language.iso en ja_JP
dc.publisher American Society for Microbiology ja_JP
dc.rights © 2018 American Society for Microbiology. ja_JP
dc.rights 出版社許諾条件により、本文は2019年4月19日以降に公開 ja_JP
dc.subject Alcoholic fermentation ja_JP
dc.subject TORC1 ja_JP
dc.subject Greatwall ja_JP
dc.subject Rim15p ja_JP
dc.subject PP2AB55δ ja_JP
dc.subject Cdc55p ja_JP
dc.subject sake yeast ja_JP
dc.subject Saccharomyces cerevisiae ja_JP
dc.subject Schizosaccharomyces pombe ja_JP
dc.title Nutrient Signaling via the TORC1-Greatwall-PP2AB55δ Pathway Responsible for the High Initial Rates of Alcoholic Fermentation in Sake Yeast Strains of Saccharomyces cerevisiae ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription ワタナベ, ダイスケ
dc.contributor.transcription タテベ, ヒサシ
dc.contributor.transcription シオザキ, カズヒロ
dc.contributor.transcription タカギ, ヒロシ
dc.contributor.alternative 渡辺, 大輔
dc.contributor.alternative 建部, 恒
dc.contributor.alternative 塩崎, 一裕
dc.contributor.alternative 髙木, 博史
dc.identifier.fulltexturl https://doi.org/10.1128/AEM.02083-18 ja_JP
dc.textversion publisher ja_JP
dc.identifier.ncid AA00543249 ja_JP
dc.identifier.jtitle Applied and Environmental Microbiology ja_JP
dc.relation.doi info:doi/10.1128/AEM.02083-18 ja_JP
dc.identifier.NAIST-ID 74650151 ja_JP
dc.identifier.NAIST-ID 73296634 ja_JP
dc.identifier.NAIST-ID 73296618 ja_JP
dc.identifier.NAIST-ID 73290561 ja_JP
dc.relation.pmid 30341081 ja_JP

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