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Computational Methods for Estimating Molecular System from Membrane Potential Recordings in Nerve Growth Cone

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dc.contributor.author Yamada, Tatsuya
dc.contributor.author Nishiyama, Makoto
dc.contributor.author Oba, Shigeyuki
dc.contributor.author Jimbo, Henri Claver
dc.contributor.author Ikeda, Kazushi
dc.contributor.author Ishii, Shin
dc.contributor.author Hong, Kyonsoo
dc.contributor.author Sakumura, Yuichi
dc.date.accessioned 2018-05-07T05:54:03Z
dc.date.available 2018-05-07T05:54:03Z
dc.date.issued 2018-03-14
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10061/12550
dc.description.abstract Biological cells express intracellular biomolecular information to the extracellular environment as various physical responses. We show a novel computational approach to estimate intracellular biomolecular pathways from growth cone electrophysiological responses. Previously, it was shown that cGMP signaling regulates membrane potential (MP) shifts that control the growth cone turning direction during neuronal development. We present here an integrated deterministic mathematical model and Bayesian reversed-engineering framework that enables estimation of the molecular signaling pathway from electrical recordings and considers both the system uncertainty and cell-to-cell variability. Our computational method selects the most plausible molecular pathway from multiple candidates while satisfying model simplicity and considering all possible parameter ranges. The model quantitatively reproduces MP shifts depending on cGMP levels and MP variability potential in different experimental conditions. Lastly, our model predicts that chloride channel inhibition by cGMP-dependent protein kinase (PKG) is essential in the core system for regulation of the MP shifts. ja_JP
dc.language.iso en ja_JP
dc.publisher Nature Publishing Group ja_JP
dc.rights © The Author(s) 2018 ja_JP
dc.title Computational Methods for Estimating Molecular System from Membrane Potential Recordings in Nerve Growth Cone ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription ヤマダ, タツヤ
dc.contributor.transcription イケダ, カズシ
dc.contributor.transcription サクムラ, ユウイチ
dc.contributor.alternative 山田, 達也
dc.contributor.alternative 池田, 和司
dc.contributor.alternative 作村, 諭一
dc.textversion author ja_JP
dc.identifier.jtitle Scientific Reports ja_JP
dc.identifier.volume 8 ja_JP
dc.identifier.issue 1 ja_JP
dc.identifier.spage 4559 ja_JP
dc.relation.doi info:doi/10.1038/s41598-018-22506-3 ja_JP
dc.identifier.NAIST-ID 82044587 ja_JP
dc.identifier.NAIST-ID 73292872 ja_JP
dc.identifier.NAIST-ID 73297863 ja_JP
dc.relation.pmid 29540815 ja_JP

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