• Structural Basis of the Sec Translocon and YidC Revealed Through X-ray Crystallography 

  Tsukazaki, Tomoya (Springer US, 2019-04)
• RNF152 Negatively Regulates mTOR Signalling and Blocks Cell Proliferation in the Floor Plate 

  Kadoya, Minori; Sasai, Noriaki (Cold Spring Harbor Laboratory, 2019-05-23)

  The neural tube is composed of a number of neural progenitors and postmitotic neurons distributed in a quantitatively and spatially precise manner. The floor plate, located in the ventral-most region of the neural tube, ...
• GPR17 is an essential regulator for the temporal adaptation of Sonic hedgehog signalling in neural tube development 

  Yatsuzuka, Atsuki; Hori, Akiko; Kadoya, Minori; Matsuo-Takasaki, Mami; Kondo, Toru; Sasai, Noriaki (Company of Biologists: Development, 2019-08-23)

  Dorsal-ventral pattern formation of the neural tube is regulated by temporal and spatial activities of extracellular signalling molecules. Sonic hedgehog (Shh) assigns ventral neural subtypes via activation of the Gli ...
• Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system 

  Urasaki, Akihiro; Morishita, Seiya; Naka, Kosuke; Uozumi, Minato; Abe, Kouki; Huang, Liguo; Watase, Emiko; Nakagawa, Osamu; Kawakami, Koichi; Matsui, Takaaki; Bessho, Yasumasa; Inagaki, Naoyuki (Springer Nature, 2019-08-21)

  The zebrafish sensory posterior lateral line is an excellent model system to study collective cell migration and organogenesis. Shootin1 is a cytoplasmic protein involved in neuronal polarization and axon guidance. Previous ...
• Cytokinin Signaling Is Essential for Organ Formation in Marchantia polymorpha 

  Aki, Shiori; Mikami, Tatsuya; Naramoto, Satoshi; Nishihama, Ryuichi; Ishizaki, Kimitsune; Kojima, Mikiko; Takebayashi, Yumiko; Sakakibara, Hitoshi; Kyozuka, Junko; Kohchi, Takayuki; Umeda, Masaaki (Oxford University Press, 2019-05-28)

  Cytokinins are known to regulate various physiological events in plants. Cytokinin signaling is mediated by the phosphorelay system, one of the most ancient mechanisms controlling hormonal pathways in plants. The liverwort ...
• Single-Unit Imaging of Membrane Protein-Embedded Nanodiscs from Two Oriented Sides by High-Speed Atomic Force Microscopy 

  Tanaka, Yoshiki; Tsukazaki, Tomoya (Elsevier, 2019-01-02)
• Structural Basis for the Function of the β-Barrel Assembly-Enhancing Protease BepA 

  Tanaka, Yoshiki; Hayashi, Yugo; Kamikubo, Hironari; Tsukazaki, Tomoya (Elsevier, 2019-02-01)

  The β-barrel assembly machinery (BAM) complex mediates the assembly of β-barrel membrane proteins in the outer membrane. BepA, formerly known as YfgC, interacts with the BAM complex and functions as a protease/chaperone ...
• 2.8-Å crystal structure of Escherichia coli YidC revealing all core regions, including flexible C2 loop 

  Tanaka, Yoshiki; Tsukazaki, Tomoya (Elsevier, 2018-10-20)

  YidC/Alb3/Oxa1 family proteins are involved in the insertion and assembly of membrane proteins. The core five transmembrane regions of YidC, which are conserved in the protein family, form a positively charged cavity open ...
• Shootin1による細胞-基質間の力の発生を介した神経細胞の細胞移動，極性形成，軸索ガイダンスおよびアクチン波 

  稲垣, 直之 (公益社団法人日本生化学会, 2019-04-25)

  Shootin1はクラッチ分子として重合・脱重合を繰り返すアクチン線維と細胞接着分子を連結することで細胞と細胞外基質の間に牽引力を生み出す．さらに，この力の発生はシグナル伝達によって調節を受ける．本稿では，最近のShootin1の解析を通じて明らかとなりつつある神経細胞の極性形成，軸索ガイダンス，細胞移動そして新たな細胞内分子輸送機構アクチン波の分子メカニクスを解説する．
• Structural Basis of Sarco/Endoplasmic Reticulum Ca2+-ATPase 2b Regulation via Transmembrane Helix Interplay 

  塚崎, 智也; 田中, 良樹 (Elsevier Inc., 2019-04-23)

  Sarco/endoplasmic reticulum (ER) Ca2+-ATPase 2b (SERCA2b) is a ubiquitously expressed membrane protein that facilitates Ca2+ uptake from the cytosol to the ER. SERCA2b includes a characteristic 11th transmembrane helix ...
• Rab33a and Rab33ba mediate the outgrowth of forebrain commissural axons 

  Huang, Liguo; Urasak, Akihiro; Inagaki, Naoyuki (Springer Nature, 2019-02-12)

  Rab small GTPases play key roles in intracellular membrane trafficking. Rab33a promotes axon outgrowth of cultured rat hippocampal neurons by mediating the anterograde axonal transport of Golgi-derived vesicles and the ...
• ERK Activity Dynamics during Zebrafish Embryonic Development 

  Wong, Kah-Loon; Akiyama, Ryutaro; Bessho, Yasumasa; Matsui, Takaaki (MDPI, 2018-12-28)

  During vertebrate development, extracellular signal-regulated kinase (ERK) is activated by growth factors such as fibroblast growth factor (FGF), and it regulates the formation of tissues/organs including eyes, brains, ...
• Editorial: Cell Adhesion and Migration in the Development of Multicellular Organisms 

  Takaaki, Matsui (Frontiers, 2018-10-22)
• Noise-resistant developmental reproducibility in vertebrate somite formation 

  Honda, Naoki; Akiyama, Ryutaro; Sari, Dini Wahyu Kartika; Ishii, Shin; Bessho, Yasumasa; Matsui, Takaaki (Public Library of Science, 2019-02-04)

  The reproducibility of embryonic development is remarkable, although molecular processes are intrinsically stochastic at the single-cell level. How the multicellular system resists the inevitable noise to acquire developmental ...
• Automatic Quantitative Segmentation of Myotubes Reveals Single-cell Dynamics of S6 Kinase Activation 

  Inoue, Haruki; Kunida, Katsuyuki; Matsuda, Naoki; Hoshino, Daisuke; Wada, Takumi; Imamura, Hiromi; Noji, Hiroyuki; Kuroda, Shinya (日本細胞生物学会, 2018-08-31)

  Automatic cell segmentation is a powerful method for quantifying signaling dynamics at single-cell resolution in live cell fluorescence imaging. Segmentation methods for mononuclear and round shape cells have been developed ...
• Chromatin-mediated feed-forward auxin biosynthesis in floral meristem determinacy 

  Yamaguchi, Nobutoshi; Huang, Jiangbo; Tatsumi, Yoshitaka; Abe, Masato; Sugano, Shigeo S.; Kojima, Mikiko; Takebayashi, Yumiko; Kiba, Takatoshi; Yokoyama, Ryusuke; Nishitani, Kazuhiko; Sakakibara, Hitoshi; Ito, Toshiro (Springer Nature, 2018-12-11)

  In flowering plants, the switch from floral stem cell maintenance to gynoecium (female structure) formation is a critical developmental transition for reproductive success. In Arabidopsis thaliana, AGAMOUS (AG) terminates ...
• A cis‐acting bidirectional transcription switch controls sexual dimorphism in the liverwort 

  Hisanaga, Tetsuya; Okahashi, Keitaro; Yamaoka, Shohei; Kajiwara, Tomoaki; Nishihama, Ryuichi; Shimamura, Masaki; Yamato, Katsuyuki T; Bowman, John L; Kohchi, Takayuki; Nakajima, Keiji (EMBO press, 2019-01-04)

  Plant life cycles alternate between haploid gametophytes and diploid sporophytes. While regulatory factors determining male and female sexual morphologies have been identified for sporophytic reproductive organs, such as ...
• Nutrient Signaling via the TORC1-Greatwall-PP2AB55δ Pathway Responsible for the High Initial Rates of Alcoholic Fermentation in Sake Yeast Strains of Saccharomyces cerevisiae 

  Watanabe, Daisuke; Kajihara, Takuma; Sugimoto, Yukiko; Takagi, Kenichi; Mizuno, Megumi; Zhou, Yan; Chen, Jiawen; Takeda, Kojiro; Tatebe, Hisashi; Shiozaki, Kazuhiro; Nakazawa, Nobushige; Izawa, Shingo; Akao, Takesh; Shimoi, Hitoshi; Maeda, Tatsuya; Takagi, Hiroshi (American Society for Microbiology, 2018-10-19)

  Saccharomyces cerevisiae sake yeast strain Kyokai no. 7 (K7) and its relatives carry a homozygous loss-of-function mutation in the RIM15 gene, which encodes a Greatwall-family protein kinase. Disruption of RIM15 in non-sake ...
• Structure-based working model of SecDF, a proton-driven bacterial protein translocation factor 

  Tsukazaki, Tomoya (Oxford University Press, 2018-04-27)

  The bacterial membrane protein SecDF enhances protein translocation across the membrane driven by the complex of SecA ATPase and SecYEG. Many newly synthesized proteins in the cytoplasm are programmed to be translocated ...
• Shootin1b Mediates a Mechanical Clutch to Produce Force for Neuronal Migration 

  Minegishi, Takunori; Uesugi, Yasuyuki; Kaneko, Naoko; Yoshida, Wataru; Sawamoto, Kazunobu; Inagaki, Naoyuki (Elsevier, 2018-10-16)

  As an essential step for brain morphogenesis, neurons migrate via mechanical interactions with components of their environment such as neighboring cells and the extracellular matrix. However, the molecular mechanism by ...

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