• A chimeric mutant analysis in yeast cells suggests BiP independent regulation of the mammalian endoplasmic reticulum-stress sensor IRE1α 

  Mai, Thanh Chi; Munakata, Takeo; Tran, Duc Minh; Takagi, Hiroshi; Kimata, Yukio (公益社団法人日本農芸化学会, 2018-05-28)

  An endoplasmic reticulum (ER)-located transmembrane protein, Ire1, triggers cytoprotective events upon ER stress. Chimeric yeast Ire1 carrying the luminal domain of the mammalian major Ire1 paralogue IRE1α is upregulated ...
• Monitoring ADP/ATP ratio in yeast cells using the fluorescent-protein reporter PercevalHR 

  Nguyen, Phuong Thi Mai; Ishiwata-Kimata, Y (公益社団法人日本農芸化学会, 2019-01-31)

  PercevalHR (Perceval High Resolution) is an artificially designed fluorescent protein, which changes its excitation spectrum based on the ADP/ATP ratio of the environment. Here we demonstrated that PercevalHR can be used ...
• The unfolded protein response alongside the diauxic shift of yeast cells and its involvement in mitochondria enlargement 

  Duc, Minh Tran; Ishiwata-Kimata, Yuki; Thanh, Chi Mai; Kubo, Minoru; Kimata, Yukio (nature, 2019-09-04)

  Upon dysfunction of the endoplasmic reticulum (ER), eukaryotic cells evoke the unfolded protein response (UPR), which, in yeast Saccharomyces cerevisaie cells, is promoted by the ER-located transmembrane endoribonuclease ...
• Categorization of endoplasmic reticulum stress as accumulation of unfolded proteins or membrane lipid aberrancy using yeast Ire1 mutants 

  Duc, Minh Tran; Takagi, Hiroshi; Kimata, Yukio (公益社団法人日本農芸化学会, 2018-10-13)

  Endoplasmic reticulum (ER)-located protein Ire1 triggers the unfolded protein response against ER-stressing stimuli, which are categorized as ER accumulation of unfolded proteins or membrane lipid-related aberrancy. Here ...
• Stress-sensing and regulatory mechanism of the endoplasmic-stress sensors Ire1 and PERK 

  Ishiwata-Kimata, Yuki; Giang, Quynh Le; Kimata, Yukio (De Gruyter, 2018-10-26)

  Ire1 and its family protein PERK are endoplasmic reticulum (ER)-stress sensors that initiate cellular responses against ER accumulation of unfolded proteins. As reviewed in this article, many publications describe molecular ...
• Pentagamavunon-1 (PGV-1) inhibits ROS metabolic enzymes and suppresses tumor cell growth by inducing M phase (prometaphase) arrest and cell senescence 

  Lestari, Beni; Nakamae, Ikuko; Yoneda-Kato, Noriko; Morimoto, Tsumoru; Kanaya, Shigehiko; Yokoyama, Takashi; Shionyu, Masafumi; Shirai, Tsuyoshi; Meiyanto, Edy; Kato, Jun-ya (Springer Nature, 2019-10-16)

  We previously showed that curcumin, a phytopolyphenol found in turmeric (Curcuma longa), targets a series of enzymes in the ROS metabolic pathway, induces irreversible growth arrest, and causes apoptosis. In this study, ...
• ABA inhibits root cell elongation through repressing the cytokinin signaling 

  Takatsuka, Hirotomo; Umeda, Masaaki (Taylor & Francis, 2019-02-09)

  Cell elongation, which plays an important role in root penetration into the soil, responds to a variety of environmental factors. A previous study demonstrated that abscisic acid, a phytohormone involved in stress responses, ...
• Cytokinin signaling coordinates development of diverse organs in Marchantia polymorpha 

  Aki, Shoiri S.; Nishihama, Ryuichi; Kohchi, Takayuki; Umeda, Masaaki (Taylor & Francis, 2019-09-18)

  Cytokinins play an essential role in plant growth and development. A recent study showed that the cytokinin signaling pathway was conserved in the liverwort Marchantia polymorpha, and that it controlled gemma cup and rhizoid ...
• Gap 2 phase: making the fundamental decision to divide or not 

  Umeda, Masaak; Shiori S, Aki; Naoki, Takahashi (Elsevier, 2019-04-05)

  The Gap phases of the cell cycle are essential to perceive internal and external signals and control cell division and differentiation. However, our knowledge of molecular mechanisms underlying G2 progression in plants ...
• Integration of transcriptional repression and Polycomb-mediated silencing of WUSCHEL in floral meristems 

  Bo, Sun; Zhou, Yingying; Cai, Jie; Shang, Erlei; Yamaguchi, Nobutoshi; Xiao, Jun; Looi, Liang-Sheng; Wee, Wan-Yi; Gao, Xiuying; Wagner, Doris; Ito, Toshiro (AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, 2019-07-01)

  Arabidopsis (Arabidopsis thaliana) floral meristems terminate after the carpel primordia arise. This is achieved through the temporal repression of WUSCHEL (WUS), which is essential for stem cell maintenance. At floral ...
• Tubulin lattice in cilia is in a stressed form regulated by microtubule inner proteins 

  Ichikawa, Muneyoshi; Khalifa, Ahmad Abdelzaher Zaki; Kubo, Shintaroh; Dai, Daniel; Basu, Kaustuv; Maghrebi, Mohammad Amin Faghfor; Vargas, Javier; Bui, Khanh Huy (National Academy of Sciences, 2019-09-16)

  Cilia, the hair-like protrusions that beat at high frequencies to propel a cell or move fluid around are composed of radially bundled doublet microtubules. In this study, we present a near-atomic resolution map of the ...
• Membrane-deformation ability of ANKHD1 is involved in the early endosome enlargement 

  Kitamata, Manabu; Hanawa-Suetsugu, Kyoko; Maruyama, Kohei; Suetsugu, Shiro (Elsevier (Cell Press), 2019-07-26)

  Ankyrin-repeat domains (ARDs) are conserved in large numbers of proteins. ARDs are composed of various numbers of ankyrin repeats (ANKs). ARDs often adopt curved structures reminiscent of the Bin-Amphiphysin-Rvs (BAR) ...
• Structural Basis of the Sec Translocon and YidC Revealed Through X-ray Crystallography 

  Tsukazaki, Tomoya (Springer US, 2019-04)
• RNF152 Negatively Regulates mTOR Signalling and Blocks Cell Proliferation in the Floor Plate 

  Kadoya, Minori; Sasai, Noriaki (Cold Spring Harbor Laboratory, 2019-05-23)

  The neural tube is composed of a number of neural progenitors and postmitotic neurons distributed in a quantitatively and spatially precise manner. The floor plate, located in the ventral-most region of the neural tube, ...
• GPR17 is an essential regulator for the temporal adaptation of Sonic hedgehog signalling in neural tube development 

  Yatsuzuka, Atsuki; Hori, Akiko; Kadoya, Minori; Matsuo-Takasaki, Mami; Kondo, Toru; Sasai, Noriaki (Company of Biologists: Development, 2019-08-23)

  Dorsal-ventral pattern formation of the neural tube is regulated by temporal and spatial activities of extracellular signalling molecules. Sonic hedgehog (Shh) assigns ventral neural subtypes via activation of the Gli ...
• Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system 

  Urasaki, Akihiro; Morishita, Seiya; Naka, Kosuke; Uozumi, Minato; Abe, Kouki; Huang, Liguo; Watase, Emiko; Nakagawa, Osamu; Kawakami, Koichi; Matsui, Takaaki; Bessho, Yasumasa; Inagaki, Naoyuki (Springer Nature, 2019-08-21)

  The zebrafish sensory posterior lateral line is an excellent model system to study collective cell migration and organogenesis. Shootin1 is a cytoplasmic protein involved in neuronal polarization and axon guidance. Previous ...
• Cytokinin Signaling Is Essential for Organ Formation in Marchantia polymorpha 

  Aki, Shiori; Mikami, Tatsuya; Naramoto, Satoshi; Nishihama, Ryuichi; Ishizaki, Kimitsune; Kojima, Mikiko; Takebayashi, Yumiko; Sakakibara, Hitoshi; Kyozuka, Junko; Kohchi, Takayuki; Umeda, Masaaki (Oxford University Press, 2019-05-28)

  Cytokinins are known to regulate various physiological events in plants. Cytokinin signaling is mediated by the phosphorelay system, one of the most ancient mechanisms controlling hormonal pathways in plants. The liverwort ...
• Single-Unit Imaging of Membrane Protein-Embedded Nanodiscs from Two Oriented Sides by High-Speed Atomic Force Microscopy 

  Tanaka, Yoshiki; Tsukazaki, Tomoya (Elsevier, 2019-01-02)
• Structural Basis for the Function of the β-Barrel Assembly-Enhancing Protease BepA 

  Tanaka, Yoshiki; Hayashi, Yugo; Kamikubo, Hironari; Tsukazaki, Tomoya (Elsevier, 2019-02-01)

  The β-barrel assembly machinery (BAM) complex mediates the assembly of β-barrel membrane proteins in the outer membrane. BepA, formerly known as YfgC, interacts with the BAM complex and functions as a protease/chaperone ...
• 2.8-Å crystal structure of Escherichia coli YidC revealing all core regions, including flexible C2 loop 

  Tanaka, Yoshiki; Tsukazaki, Tomoya (Elsevier, 2018-10-20)

  YidC/Alb3/Oxa1 family proteins are involved in the insertion and assembly of membrane proteins. The core five transmembrane regions of YidC, which are conserved in the protein family, form a positively charged cavity open ...

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