• Construction of a Quadrangular Tetramer and a Cage-Like Hexamer from Three-Helix Bundle-Linked Fusion Proteins 

  Miyamoto, Takaaki; Hayashi, Yugo; Yoshida, Keito; Watanabe, Hiroki; Uchihashi, Takayuki; Yonezawa, Kento; Shimizu, Nobutaka; Kamikubo, Hironari; Hirota, Shun (American Chemical Society, 2019-05-17)

  Self-assembled protein nanostructures have gained interest, owing to their potential applications in biomaterials; however, successful design and construction of protein nanostructures are limited. Herein, we constructed ...
• A chimeric mutant analysis in yeast cells suggests BiP independent regulation of the mammalian endoplasmic reticulum-stress sensor IRE1α 

  Mai, Thanh Chi; Munakata, Takeo; Tran, Duc Minh; Takagi, Hiroshi; Kimata, Yukio (公益社団法人日本農芸化学会, 2018-05-28)

  An endoplasmic reticulum (ER)-located transmembrane protein, Ire1, triggers cytoprotective events upon ER stress. Chimeric yeast Ire1 carrying the luminal domain of the mammalian major Ire1 paralogue IRE1α is upregulated ...
• Monitoring ADP/ATP ratio in yeast cells using the fluorescent-protein reporter PercevalHR 

  Nguyen, Phuong Thi Mai; Ishiwata-Kimata, Y (公益社団法人日本農芸化学会, 2019-01-31)

  PercevalHR (Perceval High Resolution) is an artificially designed fluorescent protein, which changes its excitation spectrum based on the ADP/ATP ratio of the environment. Here we demonstrated that PercevalHR can be used ...
• The unfolded protein response alongside the diauxic shift of yeast cells and its involvement in mitochondria enlargement 

  Duc, Minh Tran; Ishiwata-Kimata, Yuki; Thanh, Chi Mai; Kubo, Minoru; Kimata, Yukio (nature, 2019-09-04)

  Upon dysfunction of the endoplasmic reticulum (ER), eukaryotic cells evoke the unfolded protein response (UPR), which, in yeast Saccharomyces cerevisaie cells, is promoted by the ER-located transmembrane endoribonuclease ...
• Categorization of endoplasmic reticulum stress as accumulation of unfolded proteins or membrane lipid aberrancy using yeast Ire1 mutants 

  Duc, Minh Tran; Takagi, Hiroshi; Kimata, Yukio (公益社団法人日本農芸化学会, 2018-10-13)

  Endoplasmic reticulum (ER)-located protein Ire1 triggers the unfolded protein response against ER-stressing stimuli, which are categorized as ER accumulation of unfolded proteins or membrane lipid-related aberrancy. Here ...
• Stress-sensing and regulatory mechanism of the endoplasmic-stress sensors Ire1 and PERK 

  Ishiwata-Kimata, Yuki; Giang, Quynh Le; Kimata, Yukio (De Gruyter, 2018-10-26)

  Ire1 and its family protein PERK are endoplasmic reticulum (ER)-stress sensors that initiate cellular responses against ER accumulation of unfolded proteins. As reviewed in this article, many publications describe molecular ...
• Development of E-mail Delivery Mediation System Based on Interruptibility and Its Evaluation in Daily Office Work Scenario 

  Kobayashi, Yasumasa; Fujimoto, Yuichiro; Fujita, Kinya (IEEE, 2019-07-15)

  Notifications of e-mail arrivals at inappropriate times disrupt workers engaged in intellectual activities and reduce their productivity. We propose an e-mail delivery mediation system to deal with this issue. The system ...
• Robust Reflectance Estimation for Projection-Based Appearance Control in a Dynamic Light Environment 

  Akiyama, Ryo; Yamamoto, Goshiro; Amano, Toshiyuki; Taketomi, Takafumi; Plopski, Alexander; Sandor, Christian; Kato, Hirokazu (IEEE, 2019-09-10)

  We present a novel method for robustly estimating reflectance even in a dynamically changing light environment. To control the appearance of an object by using a projector-camera system, appropriate reflectance estimation ...
• Pentagamavunon-1 (PGV-1) inhibits ROS metabolic enzymes and suppresses tumor cell growth by inducing M phase (prometaphase) arrest and cell senescence 

  Lestari, Beni; Nakamae, Ikuko; Yoneda-Kato, Noriko; Morimoto, Tsumoru; Kanaya, Shigehiko; Yokoyama, Takashi; Shionyu, Masafumi; Shirai, Tsuyoshi; Meiyanto, Edy; Kato, Jun-ya (Springer Nature, 2019-10-16)

  We previously showed that curcumin, a phytopolyphenol found in turmeric (Curcuma longa), targets a series of enzymes in the ROS metabolic pathway, induces irreversible growth arrest, and causes apoptosis. In this study, ...
• A Low-Cost Interactive Writing Board for Primary Education using Distinct Augmented Reality Markers 

  Khan, Dawar; Rehman, Inam ur; Ullah, Sehat; Ahmad, Waheed; Cheng, Zhanglin; Jabeen, Gul; Kato, Hirokazu (MDPI AG, 2019-10-16)

  Educational institutions demand cost-effective and simple-to-use augmented reality systems. ARToolKit, an open-source computer tracking library for the creation of augmented reality applications that overlay virtual ...
• ABA inhibits root cell elongation through repressing the cytokinin signaling 

  Takatsuka, Hirotomo; Umeda, Masaaki (Taylor & Francis, 2019-02-09)

  Cell elongation, which plays an important role in root penetration into the soil, responds to a variety of environmental factors. A previous study demonstrated that abscisic acid, a phytohormone involved in stress responses, ...
• Cytokinin signaling coordinates development of diverse organs in Marchantia polymorpha 

  Aki, Shoiri S.; Nishihama, Ryuichi; Kohchi, Takayuki; Umeda, Masaaki (Taylor & Francis, 2019-09-18)

  Cytokinins play an essential role in plant growth and development. A recent study showed that the cytokinin signaling pathway was conserved in the liverwort Marchantia polymorpha, and that it controlled gemma cup and rhizoid ...
• Gap 2 phase: making the fundamental decision to divide or not 

  Umeda, Masaak; Shiori S, Aki; Naoki, Takahashi (Elsevier, 2019-04-05)

  The Gap phases of the cell cycle are essential to perceive internal and external signals and control cell division and differentiation. However, our knowledge of molecular mechanisms underlying G2 progression in plants ...
• Oligomerization of cytochrome c, myoglobin, and related heme proteins by 3D domain swapping 

  Hirota, Shun (Elsevier, 2019-05)

  Oligomerization of heme proteins is useful for construction of new materials with cooperative and systematic functions; thus, diverse methods have been applied for construction of artificial heme protein oligomers. ...
• Protein surface charge effect on 3D domain swapping in cells for c-type cytochromes 

  Yang, Hongxu; Yamanaka, Masaru; Nagao, Satoshi; Yasuhara, Kazuma; Shibata, Naoki; Higuchi, Yoshiki; Hirota, Shun (Elsevier, 2019-11-01)

  Many c-type cytochromes (cyts) can form domain-swapped oligomers. The positively charged Hydrogenobacter thermophilus (HT) cytochrome (cyt) c552 forms domain-swapped oligomers during expression in the Escherichia coli ...
• Determination of proton concentration at cardiolipin-containing membrane interfaces and its relation with the peroxidase activity of cytochrome c† 

  Parui, Partha Pratim; Sarakar, Yeasmin; Majumder, Rini; Das, Sanju; Yang, Hongxu; Yasuhara, Kazuma; Hirota, Shun (Royal Society of Chemistry, 2019-08-06)

  The activities of biomolecules are affected by the proton concentrations at biological membranes. Here, we succeeded in evaluating the interface proton concentration (−log[H+] defined as pH′) of cardiolipin (CL)-enriched ...
• 着桟支援のためのGNSS搬送波位相とIMUの密結合と視覚オドメトリの融合による位置推定のロバスト化 

  中村, 拓; 園部, 達也; 戸田, 裕行; 藤澤, 奈緒美; 武富, 貴史; プロプスキ, アレクサンダー; サンドア, クリスチャン; 加藤, 博一 (一般社団法人 測位航法学会, 2019)

  船舶の着桟支援を想定したロバストな高精度測位手法を開発した．本手法は，自船に固定したカメラで撮影した動画像データ，複数のGNSSアンテナから受信した民生用L1信号データ，及び，6軸IMUデータの融合により実現する．動画像データの視覚オドメトリを用いて，港湾構造物の3次元構造を復元しつつ，6軸IMUデータとGNSS衛星単位の搬送波位相の時空間変化量とのタイトカップリングによって高精度に自船の軌跡を推定する新しいオドメトリ技術を構築した．さ ...
• Conferment of CO-Controlled Dimer–Monomer Transition Property to Thermostable Cytochrome c′ by Mutation in the Subunit–Subunit Interface 

  Yamanaka, Masaru; Nakayama, Ryoko; Fujii, Sotaro; Wakai, Satoshi; Sambongi, Yoshihiro; HIrota, Shun (Chemical Society of Japan, 2019-01-11)

  Cytochrome c′ (CP) is a gas-binding homo-dimeric heme protein. Mesophilic Allochromatium vinosum CP (AVCP) and thermophilic Hydrogenophilus thermoluteolus CP (PHCP) have high sequence and structure similarities. AVCP is ...
• Construction of a Triangle‐Shaped Trimer and a Tetrahedron Using an α‐Helix‐Inserted Circular Permutant of Cytochrome c555 

  Nagao, Satoshi; Yamanaka, Masaru; Ueda, Ikki; Watanabe, Hiroki; Uchihashi, Takayuki; Shibata, Naoki; Higuchi, Yoshiki; Hirota, Shun (John Wiley & Sons, Ltd, 2018-04-16)

  Highly-ordered protein structures have gained interest for future uses for biomaterials. Herein, we constructed a building block protein (BBP) by the circular permutation of the hyperthermostable Aquifex aeolicus cytochrome ...
• Oxidative modification of methionine80 in cytochrome c by reaction with peroxides 

  Nugraheni, Ari Dw; Ren, Chunguang; Matsumoto, Yorifumi; Nagao, Satoshi; Yamanaka, Masaru; Hirota, Shun (Elsevier, 2018-05-01)

  The Met80–heme iron bond of cytochrome c (cyt c) is cleaved by the interaction of cyt c with cardiolipin (CL) in membranes. The Met80 dissociation enhances the peroxidase activity of cyt c and triggers cyt c release from ...

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