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Potential involvement of drought-induced Ran GTPase CLRan1 in root growth enhancement in a xerophyte wild watermelon

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dc.contributor.author Akashi, Kinya
dc.contributor.author Yoshimura, Kazuya
dc.contributor.author Kajikawa, Masataka
dc.contributor.author Hanada, Kouhei
dc.contributor.author Kosaka, Rina
dc.contributor.author Kato, Atsushi
dc.contributor.author Katoh, Akira
dc.contributor.author Nanasato, Yoshihiko
dc.contributor.author Tsujimoto, Hisashi
dc.contributor.author Yokota, Akiho
dc.date.accessioned 2016-07-20T01:44:46Z
dc.date.available 2016-07-20T01:44:46Z
dc.date.issued 2016-06-07
dc.identifier.issn 0916-8451
dc.identifier.uri http://hdl.handle.net/10061/10668
dc.description.abstract Enhanced root growth is known as the survival strategy of plants under drought. Previous proteome analysis in drought-resistant wild watermelon has shown that Ran GTPase, an essential regulator of cell division and proliferation, was induced in the roots under drought. In this study, two cDNAs were isolated from wild watermelon, CLRan1 and CLRan2, which showed a high degree of structural similarity with those of other plant Ran GTPases. Quantitative RT-PCR and promoter-GUS assays suggested that CLRan1 was expressed mainly in the root apex and lateral root primordia, whereas CLRan2 was more broadly expressed in other part of the roots. Immunoblotting analysis confirmed that the abundance of CLRan proteins was elevated in the root apex region under drought stress. Transgenic Arabidopsis overexpressing CLRan1 showed enhanced primary root growth, and the growth was maintained under osmotic stress, indicating that CLRan1 functions as a positive factor for maintaining root growth under stress conditions. AB - Enhanced root growth is known as the survival strategy of plants under drought. Previous proteome analysis in drought-resistant wild watermelon has shown that Ran GTPase, an essential regulator of cell division and proliferation, was induced in the roots under drought. In this study, two cDNAs were isolated from wild watermelon, CLRan1 and CLRan2, which showed a high degree of structural similarity with those of other plant Ran GTPases. Quantitative RT-PCR and promoter-GUS assays suggested that CLRan1 was expressed mainly in the root apex and lateral root primordia, whereas CLRan2 was more broadly expressed in other part of the roots. Immunoblotting analysis confirmed that the abundance of CLRan proteins was elevated in the root apex region under drought stress. Transgenic Arabidopsis overexpressing CLRan1 showed enhanced primary root growth, and the growth was maintained under osmotic stress, indicating that CLRan1 functions as a positive factor for maintaining root growth under stress conditions. ja_JP
dc.language.iso en ja_JP
dc.publisher Taylor & Francis ja_JP
dc.rights © Taylor & Francis ja_JP
dc.rights 出版社許諾条件により、本文は2017年6月7日以降公開。
dc.rights The Version of Record of this manuscript has been published and is available in Bioscience, Biotechnology, and Biochemistry, 2016.06.07, http://www.tandfonline.com/10.1080/09168451.2016.1191328.
dc.subject Drought avoidance ja_JP
dc.subject Ran-GTPase ja_JP
dc.subject Root growth ja_JP
dc.subject Wild watermelon ja_JP
dc.title Potential involvement of drought-induced Ran GTPase CLRan1 in root growth enhancement in a xerophyte wild watermelon ja_JP
dc.type.nii Journal Article ja_JP
dc.contributor.transcription アカシ, キンヤ
dc.contributor.transcription ヨシムラ, カズヤ
dc.contributor.transcription カトウ, アキラ
dc.contributor.transcription ヨコタ, アキホ
dc.contributor.alternative 明石, 欣也
dc.contributor.alternative 吉村, 和也
dc.contributor.alternative 加藤, 彰
dc.contributor.alternative 横田, 明穂
dc.identifier.fulltexturl http://dx.doi.org/10.1080/09168451.2016.1191328 ja_JP
dc.textversion author ja_JP
dc.identifier.ncid AA10824164 ja_JP
dc.identifier.jtitle Bioscience, Biotechnology, and Biochemistry ja_JP
dc.identifier.spage 1 ja_JP
dc.identifier.epage 10 ja_JP
dc.relation.doi 10.1080/09168451.2016.1191328 ja_JP
dc.identifier.NAIST-ID 73294779
dc.identifier.NAIST-ID 73290256
dc.relation.pmid 27310473 ja_JP


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